超声引导不可逆电穿孔消融山羊肝脏的实验研究

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1、超声引导不可逆电穿孔消融山羊肝脏的实验研究刘颖1，周玮1，熊正爱1，李成祥2，姚陈果2（1.重庆医科大学附属第二医院妇产科，重庆，400010；2．重庆大学输配电装备及系统安全与新技术国家重点实验室，重庆，400030）[摘要]目的探讨超声引导经皮穿刺不可逆性电穿孔（irreversibleelectroporation,IRE）微创消融山羊肝脏组织的可行性、B超影像学特点及病理分析。方法固定脉宽100μs，频率1Hz，电压2000V，脉冲数120个，彩色超声探头引导电极针经皮定位穿刺处理山羊肝脏组织，观察处理的过程中及处理后即刻、24小时靶区灰度变化，肝脏坏死

2、轮廓与仿真电场相比较，同时对应时间点取材行组织病理切片。结果超声引导经皮IRE消融靶区组织定位准确，与周围正常组织回声明显不同；光镜下观察治疗处理区肝细胞完全坏死，与周围正常细胞分界清楚；坏死组织轮廓与电极的电场仿真图像相吻合。结论超声作为一种辅助手段，实时引导定位穿刺，监测处理后坏死区回声变化范围，IRE消融范围精确、可控，为临床前期应用提供了大动物实验依据。关键词：脉冲电场；不可逆性电穿孔；超声引导；肝脏Experimentalstudyongoatliverswithultrasoundimagingguidedpercutaneousirreversib

3、leelectroporationLiuYing1,ZhouWei1,XiongZhengai1,LiChengxiang2,YaoChenguo2(1.ObstetricsandGynecologydepartment,the2ndaffiliatedhospitalofChongqingMedicalUniversity,Chongqing,400010,2.StateKeyLaboratoryofPowerTransmissionEquipment&SystemSecurityandNewTechnology;Chongqing,400010)基金项目：国

4、家自然科学基金重点项目（50637020）AbstractObjectiveToinvestigatethepropermethodofpercutaneousirreversibleelectroporation(IRE)ongoatliversunderultrasonicguidance,andobservethecharacteristicofultrasoundimagingandhistologicalfeaturechanges.MethodsPulseelectricfields(PEFs)withpermanentduration(100μs)

5、,frequency(1Hz),voltage(2000V)andpulses(120pieces)performedontheelectrodes,andplacementoftheelectrodeswasperformedunderultrasoundguidancethoughtheanimalskintothetargetarea.Thetreatedareawasobservedbyultrasoundandmicroscope,and9comparedwithfiniteelementmodeling(FEM)methodsatthetimeof0,

6、24hoursafterIREablation.ResultsUltrasoundimagingguidancewasaccuratetofocusonthetargetarea.ImagingcapturedbytheultrasoundafterIREprocedurewasquitedifferentcomparedwiththenormalliverimaging.Completehepaticcelldeathwithasharpdemarcationbetweentheablatedzoneandthenon-ablatedzonewereobser

7、ved.Necrospy-basedmeasurementdemonstratedhighlyconsistencywithFEM-anticipatedablationzones.ConclusionWithreal-timemonitoringbyultrasonographyandwell-controlledablationofthetargettissue,percutaneousIREcanprovideanovelanduniqueablativemethod.Thispaperprovidesthefundamentalexperimentalw

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