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1、单核细胞趋化蛋白-1在恶性骨肿瘤中高表达胡旭，初同伟，廖通权，余世明，刘玉刚，周跃（400037重庆，第三军医大学新桥医院骨科）［摘要］ 目的探讨单核细胞趋化蛋白-1(monocytechemotacticprotein-1,MCP-1）在人转移性骨肿瘤、恶性原发性骨肿瘤、良性骨肿瘤组织中差异性表达及其意义。方法2008年12月-2010年9月期间，收集转移性骨肿瘤15例、原发性恶性骨肿瘤15例、良性骨肿瘤15例，总共45例骨肿瘤标本。半定量RT-PCR检测各组MCP-1mRNA的表达水平。应用
2、免疫组织化学S-P法检测MCP-1蛋白的表达水平、IPP（Image-ProPlus）分析标本中阳性细胞的累积光密度值和阳性表达面积。结果转移性骨肿瘤组MCP-1mRNA相对表达量（0.862±0.042）高于恶性原发组（0.612±0.051）和良性组（0.171±0.021）（P＜0.05）。转移性骨肿瘤组织中MCP-1免疫组化累积光密度（4532.12±190.02）和阳性面积（513.32±89.08）均高于原发性恶性骨肿瘤组（2592.11±120.21）（308.34±79.33）和
3、良性骨肿瘤组（551.22±79.12）（115.91±32.68），且其在原发恶性组中的表达水平也高于良性组（P＜0.05）。结论MCP-1在恶性骨肿瘤中高表达，在转移性骨肿瘤表达更显著。［关键词］骨肿瘤；肿瘤转移；逆转录聚合酶链反应；免疫组织化学；单核细胞趋化蛋白-1[中图法分类号]R738.1[文献标志码]AHighexpressionofmMonocytechemotacticprotein-1（MCP-1）inmalignantbonetumorHuXu,ChuTongwei,Liao
4、Tongquan,YuShiming,LiuYugang,ZhouYue(DepartmentofOrthopedics,XinqiaoHospital,ThirdMilitaryMedicalUniversity,Chongqing,400037,China)［Abstract］ObjectiveToidentifyapproachtheexpressionofmonocytechemotacticprotein-1(MCP-1)inhumanmetastaticbonetumors,prim
5、arymalignantbonetumorsandbenignbonetumortissues,andthecorrelationbetweentheitsexpressionandthepathologicfeaturecharacteristicssofmetastaticbonetumor.MethodsAtotalofWecollected15tissuesamplescasesofmetastaticbonetumor,15samplesofprimarymalignantbonetu
6、morand15samplesofbenignbonetumor，totally45casesofbonetumorwerecollected.ThemRNAandproteinexpressionofMCP-1weredetectedwithRT-PCRandimmunohistochemistry,respectively.Image-ProPlusimageanalyzerwasusedtoanalyzetheintegratedopticaldensityandpositiveexpre
7、ssionarea.Accordingtothedifferentiationdegree,themetastaticbonetumorspecimensweredividedinto3groups,themRNAexpressionofMCP-1weredetected.ResultsThemRNAlevelofMCP-1washigherinmetastaticbonetumorthaninprimarymalignantbonetumorandbenignbonetumor(0.862±0
8、.042)washigherthanthatinprimarymalignantbonetumor(vs0.612±0.051and0.171±0.021,P<0.05)andbenignbonetumor(0.171±0.021)(P<0.05).ThepositiveexpressionofMCP-1proteininmetastaticbonetumor(Integratedopticaldensitywasstronglyhigherinmetastaticbonetumorthanin
