egpeife 3o 4纳米磁流体—tk对肝癌细胞的靶向性杀伤作用的体内实验研究

《egpeife 3o 4纳米磁流体—tk对肝癌细胞的靶向性杀伤作用的体内实验研究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、硕士学位论文中文摘要摘要目的：研究PEG．PEI／Fe304纳米磁流体．TK对人肝癌细胞系SMMC．7721裸鼠移植瘤模型的靶向性治疗作用。方法：建立人原发性肝癌裸鼠皮下移植瘤模型，将荷瘤裸鼠随机分成肿瘤对照组、纯自杀基因组、丙氧鸟苷(GCV)组、PEG．PEI／Fe304纳米磁流体一TK组4组。于瘤体内分别直接注射纯自杀基因、PEG．PEI／Fe304纳米磁流体．TK，同时于裸鼠腹腔内注射GCV,观察不同时段皮下肿瘤的生长情况并做病理学检查，免疫组化法检测肿瘤微血管密度(m)以及血管内皮细胞生长因子(VEGF)的表达量，原位末端标记(TUNEL)法检

2、测细胞原位凋亡。同时，测量肿瘤裸鼠治疗前后的AFP定量，并电镜下观察肿瘤细胞超微结构的变化，以及用RT-PCR检测肿瘤鼠心，肺，肾的自杀基因表达情况。结果：裸鼠皮下成瘤率100％；研究PEG．PEI／Fe304纳米磁流体一TK组的肿瘤体积、血清AFP含量、肿瘤MVD和VEGF表达强度均明显低于对照组、纯自杀基因组和GCV组(P

3、并对肿瘤治疗具有靶向性，是一种较为理想的肝脏肿瘤靶向给药系统，有望成为治疗原发性肝癌的新型生物制剂之一。关键词：肝癌，磁流体，自杀基因，PEG—PEI／Fe304纳米磁流体．TK，GCV，靶向性硕士学位论文英文摘要AbstractObjective：TostudytheeffectsofPEG—PEI／Fe304NanoparticlesMagneticFluid--TKtargetingtherapyforhu·-manprimarylivercancerinnudemiceimplantedwithSMMC-7721．Methods：Humanli

4、vercancercelllineSMMC一7721wasimplantedsubcutaneouslyinnudemicetoestablishexperimentmodel．Animalsbeatinglivercancerwererandomlydividedintofivegroups：controlgroup，GCV(ganciclovir)group，Thymidinekinasegroup；PEG—PEI／Fe304NanoparticleMagneticFluid-TK．Differentplasmidsweredirectlyinje

5、ctedintotumorsandGCVwasintrap-eritoneallyadministratedsimultaneouslyaccordingtodifferentgroups．Thegrowthoftumorswasobservedandthepathologywasexaminedaswell．SerumAFPlevelwasmeasuredbyradioimmunology,theultrastructuralchangeoftumorcellswasstudiedbyusingelectronmicroscopy,theexpres

6、sionsofMVDandVEGFwererespectivelydetectedwithimmunohistochemistryandthecellapoptosisinsituwasdetectedbyResults：ThesuccessratetoestablishsubcutaneousimplantedlivercancermodelinnudemiceWas100％．Thetumorvolume，serumAFPlevel，VEGFandMVDexpressionsofPEG-PEI／Fe304NanoparticlesMagneticFl

7、uid-TKgroupwerelowerthanthoseincontrolgroup，ThymidinekinasegroupandGCVgroup(P<0．05)andmoreapoptosiscellscouldbeobserved．TheapoptosisindexofNanoparticlesMagneticFerrofluid-TKgroupwashigherthanthatofthelatter(P<0．05)．RT-PCRIl硕七学位论文英文摘要measuredthatshowsthetumormouseheart，thelung，th

8、ekidneyhasnotseentheThymidinekinasegeneexpressi