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ID:27683189
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时间:2018-12-05
《医学毕业论文乙酰肝素酶基因表达增强大肠癌转化细胞侵袭能力的实验研究》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、&姓名:2014年6月25日乙酰肝素酶基因表达增强大肠癌转化细胞侵袭能力的实验研究【关键词】乙酰肝素酶TheEnhancedEffectofHeparanaseGeneExpressionontheInvasiveAbilityofColorectalCancerTransformedCellAbstract:ObjectiveThisstudywasinvestedtheeffectsofheparanasegeneontheinvasiveabilityofcolorectalcancercells.MethodsHeparanasegenewastra
2、nsfectedtohumancolorectalcancercelllinesHT29.ThecellgrowthkineticsandtheabilityofinvadinginvitroweredetectedbyMTTandinvadingchamber.Tumorigencityandorthotopicimplantationofhistologicallyintacttumorinnudemice’sileocecaljunctionindicatedtheinvasiveabilityofcolorectalcancertransformed
3、cellbytheexogenousexpressionofheparanasegene.ResultsAfterbeingtransfectedwiththisgene,thegrowthvelocityofthesetransfectedcellswassignificantlyrapiderthantheparentalcellline.TheBoydeninvasionchambertestweredisplayedtheinvasiveabilityoftransfectedcell(45.5土0.501)wasstrongerthannotran
4、sfectedcell(29.3土0.062)andblanktransfectedcell(30.1土0.247).Therewassignificantlydifference(PO.Ol)amongthreekindsofcells.Inthesametime,theentityneoplasmwiththetransfectedcell(12mm><9mmxlOmm)islargerthanthecontrolgroup(6mm><8mmx6mm).Theincidencesofthelivermetastases(71.43%)ishigherth
5、anthecontrolgroup(14.29%).Therateoftumorigencityandmetastasisinnudemicewassignificantlydifferentthanbeforethansfected(P0.01).ConclusionItsuggeststhathumanexogenousheparanasegenecanfacilitatetoenhancethegrowth,invasionandmetastasisincelllineHT29.Keywords:Heparanase;Colorectalneoplas
6、m;Transfection;Metastasis摘要:口的探讨外源性乙酰肝素酶基因对增强大肠癌HT29细胞转移侵袭能力的影响。力*法乙酰肝素酶(Heparanase,Hpa)基因真核表达载体转染大肠癌HT29细胞,观察转化细胞增殖和侵袭能力的变化,通过转化细胞裸鼠异种接种成瘤和实体瘤回肓部原位种植转移模型建立,观察乙酰肝素酶mRNA的表达对人肠癌HT29细胞侵袭性的影响。结果乙酰肝素酶基因转染细胞HT29Hpa较未转染细胞HT29和转染空载细胞HT29KZ生长速度明显加快;Boyden小室体外侵袭实验显示HT29Hpa细胞穿膜数(45.5士0.501)较H
7、T29细胞(29.3土0.062)和HT29KZ细胞(30.1土0.247)增多,差异具冇显著性(P0.01);转染细胞裸鼠皮卜*瘤生长速度明显加快;同期内HT29Hpa细胞形成的皮卜瘤(12mm><9mmxlOmm)较HT29细胞皮下痛(6mm><8mm><6mm)大,HT29Hpa细胞皮下瘤冋盲部原位种植致肝转移率(71.43%)较HT29细胞肝转移率(14.29%)增高。两者问差异有敁著性意义(P0.01)。结论乙酰肝素酶基因外源性的表达,能促进肿瘤细胞的生长、侵袭和转移。关键词:乙酰肝素酶;大肠癌;转染;转移0引言乙酰肝素酶(heparanase)是
8、一种广泛存在于细胞外基质、密切参与肿瘤细胞生长和侵袭
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