成年兔心耳肌细胞的分离标记与自体移植

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1、成年兔心耳肌细胞的分离标记与自体移植作者：张浩，蔡振杰，俞世强，赵璧君，张近宝【关键词】心肌/细胞学Isolation,labellingandautotransplantationofleftauriclemyocytesinadultrabbit　　【Abstract】AIM:Todevelopareliablemethodforisolationandfluorescentlabelingofadultrabbitleftauriclemyocytesandtoinvestigatethesurvival

2、ofgraftedautologousleftauriclemyocytes.METHODS:Tlyassignedtotransplantgroupandcontrolgroup(n=10).Theleftauricleofrabbitedium(controlgroup)alleftventricularanteriorensethods.RESULTS:Mostoftheisolatedcellsorphologicfeatureofthesecellscorrespondedtothatofauricl

3、ecardiomyocytesandthecellactivityyocardialinfarction(MI)areaoftransplantgroup,andthenuclEIedthesurvivalofimplantedcellsandthereliabilityofDAPIlabeling.VasculardensityintransplantgroupedigestionandDAPIlabelingarereliablemethodsfortheisolationandlabelingofleft

4、auriclemyocytesinadultrabbits.IsolatedandDAPIlabeledauriclemyocytescansurviveafterautograftedintonormalventricularanteriorayimproveperipheralvascularproliferation.　　【Keyyocardium/cytology;isolation;label;transplantation,autologous　　【摘要】目的：建立可靠的成年兔心耳心肌细胞的急性分离

5、和荧光标记的方法，观察心耳肌细胞移植到自体左室前壁的存活状况.方法：成年新西兰白兔20只，随机分为移植组和对照组（n=10）.结扎并剪取自体左心耳组织，急性消化分离为单细胞，经DAPI标记后分别将细胞悬液和培养基注射到移植组和对照组自体正常左室前壁内.4a公司）,4,6二脒基2苯茚二酮(Sigma公司).　　1.2方法　　1.2.1自体左心耳心肌组织的取材取成年兔经耳缘静脉注射戊巴比妥钠（30mg/kg），左侧胸骨旁切口，切断第4肋骨进胸，向上推开胸腺，剪开心包，显露并牵引左心耳，用4号丝线结扎左心耳基底部后，

6、剪取左心耳并立即置于预冷（4℃）的普通台式液中.用盐水纱布覆盖伤口.　　1.2.2左心耳心肌细胞的消化分离参照　　1.2.7结果观察4anpediatricandadultventricularmyocytesinculture:AssessmentofphenotypicchangesanJ,DuongM,ZibaitisA,etal.Myocardialtissueengineeringyoblastimplantation［J］.JThoracCardiovascSurg,1998;116:744-751

7、.　［5］LiRK,JiaZQ,alhotraA,LiBS,etal.Effectsofconstitutiveoverexpressionofinsulin2likegroechanicalcharacteristicsandmolecularpropertiesofventricularmyocytes［J］.CircRes,1998;82:594-603.