补肾法、疏肝法对体外培养小鼠卵泡pr调节卵泡破裂路径的影响

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1、授予单位代码10089学号或申请号20152378中国图书分类号R2-031HebeiMedicalUniversity硕士学位论文学术学位补肾法、疏肝法对体外培养小鼠卵泡PR调节卵泡破裂路径的影响研究生：刘亚华导师：杜惠兰教授专业：中西医结合临床二级学院：河北中医学院2018年3月目录中文摘要·········································································································1英文摘要···············

2、·························································································6英文缩写·········································································································13研究论文补肾法、疏肝法对体外培养小鼠卵泡PR调节卵泡破裂路径的影响前言·····································

3、·····································································14材料与方法·····························································································15结果····································································································

4、······21附图··········································································································25附表··········································································································35讨论···········································

5、·······························································42结论··········································································································53参考文献······························································································

6、····54综述排卵障碍性不孕的中医药治疗策略··················································59致谢·················································································································68个人简历·············································································

7、·····························70中文摘要补肾法、疏肝法对体外培养小鼠卵泡PR调节卵泡破裂路径的影响摘要目的：针对排卵障碍为女性不孕症的主要原因这一问题，本课题组在前期研究证实补肾法、疏肝法均可调节卵巢功能、促进卵泡发育、诱发排卵的基础上，研究补肾法、疏肝法诱发排卵与孕激素受体（progestronereceptor,PR）调节卵泡破裂路径的关系，以期揭示补肾法、疏肝法诱发排卵的作用及其机制，丰富中医生殖理论。方法：1.大鼠含药血清制备取40只6周龄健康雌性SD大鼠，制备得补肾调经Ⅱ号方高剂量、补肾调经Ⅲ号方高剂

8、量、逍遥丸高剂量、逍遥丸低剂量含药血清和正常大鼠血清。2.窦前卵泡的体外培养选用出生D10的昆明乳鼠，随机分为4组：空白组、正常组、补肾组、疏肝组。采用机械法分离窦前卵泡。三气培