膝骨关节炎患者关节液中IL17表达水平的临床研究.doc

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1、膝骨关节炎患者关节液中IL17表达水平的临床研究作者：董军峰,郑之和,李新志,卢国强,曾勇【摘要】目的探讨膝骨关节炎(kneeosteoarthritis,KOA)患者膝关节液中IL17表达水平。方法用双抗体夹心ELISA法测定37例(41膝)KOA患者与24例(24膝)志愿者膝关节液中IL17浓度并比较两组差异。将KOA患者按综合评分法分为轻(13膝)、中(19膝)、重(9膝)三组，比较三组患者膝关节液中IL17浓度的差异。结果KOA患者膝关节液中IL17浓度为156.76±112.78pg/ml，明显高于对照组31.67±18.

2、74pg/ml（P<0.01）。轻、中、重三组患者膝关节液中IL17的浓度分别为50.16±24.15pg/ml、154.63±47.39pg/ml、314.83±105.89pg/ml，呈现出随着病情分级逐渐加重而IL17表达水平逐渐增高的趋势(P<0.01)。结论IL17作为一种前炎症细胞因子，参与了KOA疾病的诱发、触发了病理改变、加快了病情的进展。IL17可作为判断KOA临床病情、预后的免疫学指标之一。对IL17的研究将有助于进一步在分子水平阐明KOA的发病机制，为临床诊断和治疗KOA提供新的思路。【关键词】膝骨

3、关节炎；IL17；表达；ELISA【Abstract】ObjectiveToexploretheexpressionoftheIL17inthekneeosteoarthritis(KOA)patients’sickkneejointsynovia.MethodsTheconcencentrationsoftheIL17inthekneejointsynoviaweredetectedbytwoantibodiessandwichELISAmethodandcomparedbetweenthe37cases(41knees)ofthe

4、KOApatientsand24cases(24knees)ofvolunteers.TheKOApatientsweredividedintothemildgroup(13knees),themoderategroup(19knees)andtheseveregroup(9knees).TheconcentrationsoftheIL17inthekneejointsynoviawerecomparedinthethreegroups.ResultsTheconcentrationoftheIL17inthesickkneejoints

5、ynoviaofthesickgroupwas156.76±112.78pg/ml,whichwassignificantlyhigherthanthecontrastgroup(31.67±18.74pg/ml)(P<0.01).Theconcentrationofthesickkneejointsynoviaofthemildgroup,moderategroupandseveregroupwere50.16±24.15pg/ml,154.63±47.39pg/mland314.83±105.89pg/ml,respectively

6、,whichappearedagradualelevatoryuptrendinthestateofillness’development(P<0.01).ConclusionsIL17participatestheKOA’sevocation,triggesthealterationinpathology,speedsuptheprogressionofthepathogeneticcondition,asapreinflammationfactor.IL17canbeanimmunologicalindexofjudgingt

7、heclinicalpathogeneticconditionsandprognosisfortheKOApatientstoofferanewideatothediagnosisandtherapyofKOA.8【Keywords】kneeosteoarthritis(KOA),Interlekin17,expression,ELISA膝骨关节炎(kneeosteoarthritis,KOA)是一种慢性进行性骨关节疾病,以关节软骨退行性变为主要病理特征,其早期X线征象不明显。通过检测血清、关节液或尿液中某些能间接反映关节软骨代谢状况的特异

8、性分子,有助于判断OA软骨病变程度、预后和评价药物疗效,有望成为KOA早期诊断的有效辅助方法。本研究采用双抗体夹心酶联免疫吸附法(ELISA)定量测定对照组及KOA