膝骨关节炎患者关节液中IL17表达水平的临床研究【临床医学论文】.doc

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1、临床医学论文•膝骨关节炎患者关节液中IL17表达水平的临床硏究作者：董军峰，郑之和，李新志，卢国强，曾勇【摘要】目的探讨膝骨关节炎（kneeosteoarthritis,KOA）患者膝关节液中IL17表达水平。方法用双抗体夹心ELISA法测定37例⑷膝）K0A患者与24例（24膝）志愿者膝关节液中IL17浓度并比较两组差异。将K0A患者按综合评分法分为轻（13膝）、中（19膝）、重（9膝）三组，比较三组患者膝关节液中IL17浓度的差异。结果K0A患者膝关节液中IL17浓度为156.76±112.78pg/ml，明显高于对照

2、组31.67±18.74pg/ml（P<0.01）。轻、中、重三组患者膝关节液中IL17的浓度分别为50.16±24.15pg/ml、154.63±47.39pg/ml、314.83+105.89pg/ml呈现出随着病情分级逐渐加重而IL17表达水平逐渐增高的趋势（P<0.01）。结论IL17作为一种前炎症细胞因子，参与了K0A疾病的诱发、触发了病理改变、力口快了病情的进展。IL17可作为判断K0A临床病情、预后的免疫学指标之一。对IL17的硏究将有助于进一步在分子水平阐明K0A的发病机制，为临床诊断和治疗K0A提供新的思

3、路。【关键词】膝骨关节炎；IL17；表达；ELISA[Abstract】ObjectiveToexploretheexpressionoftheIL17inthekneeosteoarthritis（KOA）patients，sickkneejointsynovia.MethodsTheconcencentrationsoftheIL17inthekneejointsynoviaweredetectedbytwoantibodiessandwichELISAmethodandcomparedbetweenthe37casc

4、s(41knees)oftheKOApatientsand24cascs(24knees)ofvolunteers・TheKOApatientsweredividedintothemiIdgroup(13knees),themoderategroup(19knees)andtheseveregroup(9knees)・TheconcentrationsoftheIL17inthekneejointsynoviawerecomparedinthethreegroups.ResultsTheconcentrationofthe

5、IL17inthesickkneejointsynoviaofthesickgroupwas156・76±112.78pg/ml,whichwassignificantlyhigherthanthecontrastgroup(31.67±18.74pg/ml)(PcO.Ol).TheconcentrationofthesickkneejointsynoviaofthemiIdgroup,moderategroupandseveregroupwere50.16±24.15pg/ml,154.63±47.39pg/mland3

6、14.83±105.89pg/ml,respectively,whichappearedagradualelevatoryuptrendinthestateofi1lness*development(P<0.01)・ConclusionsIL17participatestheKOA'sevocation,triggesthealterationinpathology,speedsuptheprogressionofthepathogeneticcondition,asapreinflammationfactor.IL17c

7、anbeanimmunologicalindexofjudgingtheclinicalpathogeneticconditionsandprognosisfortheKOApatientstoofferanewideatothediagnosisandtherapyofKOA.[Keywords】kneeosteoarthritis(KOA),Inter1ekin17,expression,ELISA膝骨关节炎(kneeosteoarthritis,KOA)是一种慢性进行性骨关节疾病，以关节软骨退行性变为主耍病理特征,其

8、早期X线征象不明显。通过检测血清、关节液或尿液中某些能间接反映关节软骨代谢状况的特异性分子，有助于判断0A软骨病变程度、预后和评价药物疗效，有望成为KOA早期诊断的有效辅助方法。本硏究采用双抗体夹心酶联免疫吸附法(ELISA)定量测定对照组及KOA患者关节液中IL17(interleukin17，IL1