电针联合西乐葆缓解大鼠胫骨癌痛.doc

《电针联合西乐葆缓解大鼠胫骨癌痛.doc》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、电针联合西乐葆缓解大鼠胫骨癌痛作者：毛应启梁,任典寰,米文丽,刘琼,王彦青【摘要】　　目的：建立大鼠胫骨癌痛模型，观察电针和西乐葆对大鼠胫骨癌痛的缓解作用。方法：采用经皮穿刺技术将大鼠乳腺癌细胞Walker256接种至雌性Wistar大鼠胫骨骨髓腔内，建立大鼠胫骨癌痛模型。大鼠造模后，采用电针和西乐葆灌胃治疗，观察其对大鼠骨肿瘤引起的机械性痛觉超敏的影响。结果：模型组大鼠后肢逐渐产生机械性痛觉超敏，接种后第16天胫骨近心端可见显著的肿瘤生长。单用电针或西乐葆5mg/(kg·d)治疗大鼠，与模型组相比其机械性疼痛阈值差异无统计学意义；

2、治疗后第22天和第26天，西乐葆10mg/(kg·d)组大鼠后肢机械性疼痛阈值较溶剂对照组提高（P<0.05）；治疗后第10、18和23天，电针合用西乐葆5mg/(kg·d)组大鼠机械性疼痛阈值高于模型组，差异有统计学意义（P<0.05）。结论：电针与小剂量西乐葆具有协同作用，合用可以增强对大鼠胫骨癌痛的镇痛效果。【关键词】骨癌痛电针西乐葆镇痛大鼠　　Objective:Toestablishaproperexperimentalmodelofbonecancerpaininratforacupunctureresear

3、ch,andobservethepainrelievingeffectofelectroacupuncture(EA)and/orCelebrexonbonecancerpaininrats.　　Methods:TheratmodelofbonecancerpainwasestablishedbypercutaneousdirectpuncturetechniqueandinoculatingtheratmammaryglandcarcinomacellsWalker256intotibialmedullarycavitydire

4、ctly,andevaluatedbydetectingthebonetumorgrowthandmechanicalallodynia.TheeffectsofdailyEAtreatmentand/orCelebrextreatmentontheratmechanicalallodyniaafterintratibialWalker256inoculationwasobservedinthisstudy.　　Results:Significantmechanicalallodyniainipsilateralhindpawand

5、tumorgrowthinproximalendoftibialboneofratsintheuntreatedgroupwereobservedafterintratibialWalker256inoculation.ThemechanicalallodyniathresholdsinratsthatreceivedEAor5mg/(kg·d)Celebrextreatmentshowednosignificantdifferenceascomparedwiththatofratsintheuntreatedgroup.Howev

6、er,themechanicalallodyniathresholdsofratsin10mg/(kg·d)Celebrexgroupshowedsignificantincreaseafter22and26daytreatmentascomparedwiththatinthemethylcellulose(MC)group.TherewassignificantdifferencebetweenratswithEAcombinedwith5mg/(kg·d)Celebrextreatmentandratsintheuntrea

7、tedgroupafter10,18and23daytreatment.9　　Conclusion:EAand5mg/(kg·d)Celebrexhavesynergisticeffectonpainrelievingandtheircombinedusemayenhancetheanalgesiceffectonbonecancerpain.　　Keywords:bonecancerpain;electroacupuncture;Celebrex;analgesia;rats　　癌痛是临床上常见的慢性病理性疼痛，严重影响了肿

8、瘤患者的生活质量。研究表明，针刺和环氧合酶2（cyclooxygenase2,COX2）抑制剂有缓解癌痛作用［13］，但有一定的局限性。针药结合是提高镇痛效应的重要途径［4］，不仅能减少药物用量及不良反应，还能有效克服针刺镇痛效果