重组人生长激素对体外培养成骨细胞的影响

《重组人生长激素对体外培养成骨细胞的影响》由会员上传分享，免费在线阅读，更多相关内容在工程资料-天天文库。

1、牙体牙髓牙周病学杂志(ChinJConservDent)2005,15(8)重组人生长激素对体外培养成骨细胞的影响・443・locatedintheTomb,DongShenJiabang,deferthenextdayfocusedontheassassination.Linping,Zhejiang,1ofwhichliquorwinemasters(WuzhensaidinformationisCarpenter),whogotAfewbayonets,duetomissedfatal,

2、whennightcame1112locatedintheTomb,DongShenJiabang,deferthenextdayfocusedontheassassination.Linping,Zhejiang,1ofwhichliquorwinemasters(WuzhensaidinformationisCarpenter),whogotAfewbayonets,duetomissedfatal,whennightcame(1.西安交通大学口腔医院,710004;2.第四军医大学口腔医

3、学院,陕西西安710032)[摘　要]　　目的:观察重组人生长激素对体外成骨细胞直接、期的影响。方法:将不同浓度的重组人生长激素(recombinanthumangrowthhormone,RHGH)加入大鼠成骨细胞培养体系,观察不同作用时间点对大鼠成骨细胞的增殖、性磷酸酶活性及细胞周期的影响。结果:RHGH对成骨细胞增殖、碱性磷酸酶活性均有影响,它可促进成骨细胞增殖,提高其碱性磷酸酶活性,且可促使细胞周期中S期、G2/M期细胞百分比增加,其作用强度随浓度增加有增强趋势。结论:RHGH对成骨细胞

4、的合成代谢有直接影响,对成骨细胞的增殖、性磷酸酶活性影响远强于雌激素。[关键词]成骨细胞;重组人生长激素;细胞增殖[中图分类号]R780.2　　　[文献标识码]A　　　[文章编号]1005-2593(2005)08-0443-04[牙体牙髓牙周病学杂志,2005,15(8):443]EffectofrecombinanthumangrowthhormoneincultivatedosteoblastsofratsinvitroSHIYong,LIXiao-hong,GUOSan-ping,SON

5、GWei(CollegeofStomatology,Xi′JiaotongUniversity,Xi′71004,China)[Abstract]　　AIM:Toinvestigatethedirect,shorttermeffectsofrecombinanthumangrowthhormone(RHGH)entdosesindifferenttime,andthencellproliferation,theactivityofalkalinephosphatase(ALP)andcellcy

6、clewereobserved.RESULTS:RHGHstimulateproliferationandALPactivityofosteoblasts.Atthesametime,SphaseandG2/Mphasepercentagewassignificantlyhigherascomparedwiththecontrol.Theeffectstendedtogetstrongerastheconcentrationsincreased.CONCLUSION:Theresultsobst

7、ainedinthisstudysuggestthatrh-ghexertsdirectanaboliceffectsonosteoblasts.Theeffectsofrh-ghwerehigherthantheestrogen.[Keywords]osteoblasts;recombinanthumangrowthhormone;cellproliferation[ChineseJournalofConservativeDentistry,2005,15(8):443]　　生长激素是垂体分泌

8、的一种促进生长发育的临床实验,均发现生长激素对骨代谢有重要作[3-5]发育迟缓、术或创伤恢复期、老年人免疫功能低同浓度的RHGH对体外培养成骨细胞的增殖、分[1]素(recombinanthumangrowthhormone,RHGH)对研究提供理论依据。[2]生长激素应用于骨代谢疾病的治疗进行了大量的1.1　材料收稿日期:2005-04-05;修回日期:2005-06-14locatedintheTomb,DongShenJiabang,deferthenextdayfocusedonthea