复方参七汤对人结肠癌细胞株裸鼠肝转移影响.doc

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1、复方参七汤对人结肠癌细胞株裸鼠肝转移影响作者：陈瑞新,徐青,于秀,陈玉泉,沈洪薰　[摘要]　　目的:建立人结肠癌细胞株裸鼠肝转移模型，研究复方参七汤对裸鼠肝转移模型的影响。方法:采用BALB/C裸鼠，Ls174t人大肠癌细胞株,脾脏切除脾脏种植法建立裸鼠肝转移模型。给予复方参七汤颗粒饲料喂养，观察体态及肿瘤生长情况；采用巢式逆转录聚合酶链反应（RT-PCR）检测裸鼠循环血中CK20mRNA表达；标本作病理组织学检查。结果:①脾脏切除脾脏种植法建立裸鼠结肠癌肝转移模型的成功率为100%，病理组织学证实肝转移癌细胞呈人结肠癌低分化腺癌特征；②实

2、验组裸鼠肿瘤数目及重量明显低于对照组，P<0.05；③实验组裸鼠肝转移血中CK20mRNA表达为0.398±0.143,明显低于对照组0.518±0.090，P<0.05。结论:复方参七汤可显著降低肝转移裸鼠血中CK20mRNA表达，抑制裸鼠结肠癌肝转移的发生及发展，可作为大肠癌术后预防复发和肝转移治疗的手段之一。　　[关键词]　　结肠癌;肝转移;裸鼠;CK20mRNA;RT-PCR　　Abstract:ObjectiveToestablishthelivermetastasesofcolonicadenocarcinomafo

3、rexperimentalstudyoftheeffectofcompoundshenqitanginnudemice.MethodsNudemicelivermetastasismodelofcoloniccancerwasestablishedwithhumancoloniccancercellsline(Ls174t)inoculatedintomicespleen.Granularforagewithcompoundshenqitangwasfedinexperimentalgroupandgranularforagewithout

4、compoundshenqitangwasfedincontrolgroupdaily.Theweightandsizeofthemiceandgrowthofthecarcinomawererecorded.Allspecimenswereexaminedhistologically.CK20mRNAinbloodinnudemicewithlivermetastasisofcoloniccarcinomawasdetectedwithnestedRT-PCR.ResultsTheincidenceoflivermetastasiswas

5、100%inthisintrasplenicinjectionmodel.Thepathologicalresultsshowedthattumourcellsoflivermetastaseswaspoorlydifferentiatedhumancolonicadenocarcinoma.Inexperimentalgroup,thetumornumberandtheweightoflivermetastasesweresignificantlylowerthanthoseincontrolgroup(P<0.05).Usings

6、emiquantitativeexamination,inexperimentalgrouptherelativevalueofCK20mRNAexpressioninblood(0.40±0.14)wassignificantlylowerthanthat(0.52±0.09)incontrolgroup(P<0.05).ConclusionCompoundshenqitangcouldeffectivelyinhibittheoccurrenceoflivermetastasiscarcinomaanddecreasethepos

7、itiveexpressionofCK20mRNAinexperimentalgroup,suggestingthatcompoundshenqitangmaybecomethepotentialtherapeuticstrategyforlivermetatstasisofcoloniccancer.6　　Keywords:Coloniccarcinoma；Livermetastases；Nudemice；CK20mRNA；RT-PCR　　大肠癌是临床最常见的恶性肿瘤之一，目前仍以手术切除为首选方法，但由于术后局部复发和转移，其整体治疗水

8、平仍不尽人意，术后5年生存率仍在50%~55%之间[1-2]。近年来，通过西医和中医药的综合治疗，患者的生存率有了明显提高[3]。本实验通过建立裸鼠肝转移模型，应用CK20mRN