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ID:17486728
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时间:2018-09-02
《Na+H+交换体1反义基因磁性纳米微粒对SGC7901胃癌细胞形态学的影响.doc》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、Na+H+交换体1反义基因磁性纳米微粒对SGC7901胃癌细胞形态学的影响作者:刘海峰陈刚滕小春汪兴伟【摘要】目的探讨Na+H+交换体1(Na+H+exchanger1,NHE1)反义基因磁性纳米微粒对SGC7901胃癌细胞形态学的影响。方法构建NHE1反义基因真核表达载体和NHE1反义基因磁性纳米微粒,将NHE1反义基因转染至SGC7901胃癌细胞中,从光镜、电镜水平观察转染与未转染细胞形态学的变化。结果氧化铁磁性纳米颗粒成功地将NHE1反义基因转染至SGC7901胃癌细胞中。与亲本细
2、胞相比,光镜下NHE1反义基因转染的SGC7901胃癌细胞胞体增大,胞质丰富,核大小相对一致,核质比和核分裂象减少,异型性减少。透射电镜下细胞线粒体数量增多、体积增大,并可见髓鞘样变,粗面内质网、高尔基体较发达,内质网扩张,核偏向一侧,极性增强。结论NHE1反义基因磁性纳米微粒能使SGC7901胃癌细胞发生形态学及超微结构的变化,有促进SGC7901胃癌细胞分化的作用。【关键词】Na+H+交换体1;磁性纳米微粒;反义基因治疗;胃肿瘤【Abstract】ObjectiveTostudytheef
3、fectsofNa+H+exchanger1(NHE1)antisensegenemagneticnanoparticiesonthemorphologyofSCG7901gastriccanercells.MethodsAntisenseNHE1eukaryoticexpressiononvectorpcDNA3.1wasconstructedwithrecombinantDNAtechniqueandtransfectedintoSGC7901gastriccarcinomacellsbyD
4、CIONP.Thedextrancoatedironoxidenanoparticles(DCIONP)wassynthesizedwithdeposition.Then,NHE1antisensegenemagneticnanoparticleswereconstructed.Weexaminedthemorphologicalchangesofthetransfectedanduntransfectedcellsunderlightandelectronmicroscopes.ResultsDCI
5、ONPsuccessfullytransfectedNHE1antisensegeneintoSGC7901gastriccancercells.Underthelightmicroscope,wefoundthattransfectedSGC7901gastriccancercellswithNHE1antisensegenewerelargerwithabundantcytoplasmandcomparativelysamesizeofnuclei,andtheratioofnucleitoc
6、ytoplasm,mitoticcountandheteromorphismdecreasedwhentheywerecomparedwiththeirparentcells.TheelectronmicroscopeshowedincreasednumberofmitochondrionandsizeofthetransfectedSGC7901gastriccancercellswithNHE1antisensegene,expandedGolgiapparatus,roughendoplasm
7、icreticulumandendoplasmicreticulumandstrengthenedheteropolarity.ConclusionThemorphologyandmicrostructureofSGC7901gastriccarcinomacellschangeafterthetransfectionofantisenseNHE1genethatcanpromotethedifferentiationofSGC7901gastriccarcinomacells.6【Keyword
8、s】Na+H+exchanger1;Magneticnanoparticles;Antisensegenetherapy;Gastriccancer传统的基因治疗方法大都缺乏靶向性,严重阻碍了基因治疗研究的快速发展。开发新的基因载体是增强基因治疗靶向性的关键问题。纳米技术的发展,为解决基因转移载体问题提供了新的思路。体积微小、具有超顺磁性的氧化铁磁性纳米颗粒,有望成为理想的基因载体材料。胞膜离子交换蛋白Na+H+交换体1(Na+H+exchange
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