表皮生长因子改善移植小肠黏膜屏障功能的实验研究.doc

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1、表皮生长因子改善移植小肠黏膜屏障功能的实验研究作者：李可洲伍晓汀　李宁黎介寿【摘要】目的了解表皮生长因子(epidermalgrowthfactor，EGF)对移植小肠通透性及细菌易位的作用。方法以Wistar大鼠为供体，SD大鼠为受体行异位全小肠移植，并以环孢素A(CsA，6mg·kg-1·d-1，im)抑制排斥反应。表皮生长因子组(EGF组)用微量输液泵持续均匀输入EGF200μg·kg-1·d-1；对照组输入等量生理盐水。第7天以乳果糖及甘露醇行移植肠灌注并收集尿液行高效液相色谱仪分析乳果糖及甘露醇含量，第

2、8天采集移植肠系膜淋巴结及门静脉血行细菌培养。结果对照组尿液中乳果糖含量［(0.093±0.008)vs(0.015±0.002)，P=0.0001］及乳果糖/甘露醇比值［(0.132±0.021)vs(0.020±0.005)，P=0.0001］明显高于基准，EGF组乳果糖含量［(0.043±0.008)vs(0.015±0.002)，P=0.0054］及乳果糖/甘露醇比值［(0.060±0.017)vs(0.020±0.005)，P=0.0029］也较基准增加，EGF组乳果糖含量［(0.043±0.008)v

3、s(0.093±0.008)，P=0.0067)及乳果糖/甘露醇比值［(0.060±0.017)vs(0.132±0.021)，P=0.0116］显著低于对照组。EGF组移植肠系膜淋巴结细菌阳性率为10%，对照组阳性率为60%，明显高于EGF组(P=0.028)。EGF组与对照组门静脉血培养阳性率比较差异无统计学意义(P>0.05)。结论本研究提示EGF能够降低同种移植小肠的通透性及细菌易位率，改善肠黏膜屏障功能。【关键词】表皮生长因子黏膜屏障功能小肠移植通透性细菌易位【Abstract】Objective

4、Toevaluatetheeffectofepidermalgrowthfactor(EGF)inpermeabilityandbacterialtranslocationaftersmallboweltransplantationinrats.MethodsHeterotopicintestinefromWistarratswastransplantedintoSDrats,withcyclosporineA(CsA)at6mg/kgperday,im,tosuppressrejectreaction.EGFg

5、roupwasinjectedsubcutaneouslywithEGFat200μg/kgperday.Controlgroupwasonlyinjectedwithnormalsaline.At7thday,thetransplantedintestineswereinfusedwithlactuloseandmannitoltocollecturineforanalyzingcontentoflactuloseandmannitolbyhighperformanceliquidchromatogram.Wh

6、ileat8thday,bloodfrommesentericlymphnodeandportalveinwascollectedforbacteriumculture.ResultsLactulosecontentinurinewas0.093±0.008(basevalue:0.015±0.002,P=0.0001)incontrolgroupand0.043±0.008(basevalue:0.015±0.002,P=0.0054)inEGFgroup,whichweresignificantlyhighe

7、rthanbasevalue.Lactose/Manitol(L/M)was0.132±0.021(basevalue:0.020±0.005,P=0.0001)incontrolgroupand0.060±0.017(basevalue:0.020±0.005,P=0.0029)inEGFgroup,whichweresignificantlyhigherthanbasevalue.5Lactulosecontent(0.043±0.008)inEGFgroupwassignificantlylowerthan

8、0.093±0.008incontrolgroup(P=0.0067)andL/M(0.060±0.017)inEGFgroupsignificantlylowerthan0.132±0.021incontrolgroup(P=0.116).Positiverateofbacterialcultureinmesentericlymphwas10%inEGFgroup,60