去卵巢大鼠海马及额叶皮层海马胆碱能神经刺激肽含量的变化

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1、去卵巢大鼠海马及额叶皮层海马胆碱能神经刺激肽含量的变化作者：高飞宋士军胡进中李芳芳张若楠【摘要】目的观察雌激素缺乏对大鼠海马CA1/CA2区及额叶皮层海马胆碱能神经刺激肽（HCNP）含量的影响。方法选取清洁级4月龄雌性SD大鼠30只，随机分为3组：假手术组（Sham组）、去卵巢组（OVX组）和去卵巢加雌二醇（E2）组（OVX+E2组），每组10只。除Sham组做假性手术外，其余2组均行双侧卵巢切除术；OVX+E2组去卵巢手术10d后给予E2皮下注射，100μg/kg，隔日1次。去卵巢手术后常规喂养4个月，获取组织标本，用于组织蛋白和组织总RNA的提取。用Western印迹法测定大

2、鼠海马和皮层CHCNPpp、NHCNPpp的相对含量。用RTPCR技术检测HCNPppmRNA水平。结果OVX组大鼠海马和皮层中CHCNPpp的相对含量较Sham组和OVX+E2组明显升高(P＜0.05)，而Sham组和OVX+E2组之间无显著差别(P＞0.05)，三组大鼠之间的海马和皮层中NHCNPpp的相对含量差异不显著(P＜0.05)；OVX组和OVX+E2组大鼠HCNPppmRNA表达与Sham组无显著差异(P＞0.05)，OVX+E2组大鼠HCNPppmRNA表达与OVX组相比增高(P＜0.05)；三组大鼠皮层HCNPppmRNA表达无显著差异(P＞0.05)

3、。结论雌激素缺乏可导致大鼠海马CA1/CA2区及额叶皮层HCNP的增多。【关键词】11去卵巢；雌激素；海马；额叶皮层；海马胆碱能神经刺激肽（HCNP）【Abstract】ObjectiveToobservetheeffectofthedefectofestrogenonthecontentsofHCNPinregionsofCA1/CA2ofhippocampusandtheexpressionofHCNPppmRNAinprefrontalcortexofovariectomized（OVX）SDrats.MethodsThirtyfemalecleanSDratsaged4m

4、onthswererandomlydividedintosham,OVX,OVXandestradiol(OVX+E)groups,andtenratspergroup.Shamgroupwasablatedsomeadiposetissueaboveovaries,OVXgroupwasablatedtwosidesovaries,OVX+Egroupwassuppliedbyestradiol(100μg/kg,qod)after10dofovariectomy.Postoperativeratswerefedbyroutinemethodandthensacrificed4

5、monthslatertoobtaintissuesandspecimenfortheuseoftheextractionoftissues’proteinandtotalRNA.TherelativeproteincontentsofCHCNPppandNHCNPppweremeasuredbyWesternblotting,andtheexpressionofHCNPppmRNAwasmeasuredbyRTPCRinthedorsalhippocampusandprefrontalcortexofovariectomizedrats.ResultsComparedwi

6、ththatofshamgroupandOVX+Egroup,therelativecontentofCHCNPppofOVXgroupinhippocampusandprefrontalcortexwashigher,andtherewasnotdifferentbetweenshamgroupandOVX+Egroup(P>0.05).However，theexpressionofNHCNPppinhippocampus11andprefrontalcortexwasnotdifferentamongthreegroups(P>0.05).Comparedwi

7、ththatofshamgroup,therelativeexpressionofHCNPppofOVXgroupandOVX+Egroupinhippocampuswasnotdifferent(P>0.05).ComparedwiththatofOVXgroup,therelativeexpressionofHCNPppmRNAofOVX+Egroupinhippocampuswashigher(P＜0.05).TherelativeexpressionofHCNPpp