il1β、il6、tnfα在兔腰椎软骨终板退变中的变化及作用

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1、IL1β、IL6、TNFα在兔腰椎软骨终板退变中的变化及作用作者：刘斌，金大地，瞿东滨，蔡道章【摘要】［目的］研究IL1β、IL6、TNFα在软骨终板退变中的作用。［方法］新西兰白兔24只随机分为实验组与对照组。实验组通过腰椎失稳而诱导了腰椎软骨终板退变动物模型。分别于术后12、24、36周摄腰椎X线片及扫描电镜观察，检测不同时段软骨终板中IL1β、IL6、TNFα含量。［结果］X线片示实验组较对照组钙化明显；Bradner指数示椎间隙高度随时间延长而逐渐降低；扫描电镜示实验组软骨终

2、板随时间延长胶原纤维逐渐变细、排列紊乱、出现裂隙，蛋白多糖丢失加剧，上述变化较对照组显著；实验组IL1β、IL6、TNFα含量较对照组增多（P＜0.05）。［结论］IL1β、IL6、TNFα在软骨终板退变过程中含量相应增加，提示炎性细胞因子可能在软骨终板退变的形成和发展中起重要作用。【关键词】炎性细胞因子；腰椎；软骨终板；退变　　RoleandchangeofIL1β,IL6andTNFαduringrabbitlumbarcartilageendplatedegeneration∥

3、　　Abstract：［Objective］Tostudytheroleofnitricoxide14onthedegenerationofthecartilageendplate(CEP).［Method］TwentyfourNewZealandrabbitsweredividedintoexperimentandcontrolgroups.Intheexperimentgroup,themodeloflumbarCEPdegenerationwasestablishedbyresectionof

4、alllumbarsupraspinousandinterspinousligaments,excisionofpartsofzygapophysialjointsanddetachmentoftheposteriorparavertebralmusclesfromlumbarvertebraes.MechanicalinstabilityofthelumbarspinecouldinducedtheprocessofCEPdegeneration.TheXrayexaminationoflumba

5、rspinewasexaminedbyat12,24and36weekswithorwithoutoperation,respectively.ChangesoftheultrastructureofCEPwerealsoobservedbyscanningelectronmicroscope(SEM)duringthisprocess,andthecontentofIL1β,IL6,TNFαintheCEPwerealsodetectedduringtheperiods.［Result］Xr

6、aygraphshowedthattheCEPcalcifiedgraduallywithtimeincreasing,andthoseoftheexperimentgroupscalcifiedmoreobviouslythanthoseofthecontrolgroups.TheSEMshowedthatcollagenfibersbecamethin,irregularandfissuredandthecontentofproteoglycandecreasedseverelywiththeel

7、apseoftime.Thechangesinexperimentgroupsweremoreobviousthanthoseincontrolgroups.ThecontentofIL1β,IL6,TNFαhadasignificantdifferencebetweentheexperimentgroupsandthe14controlgroups(P＜0.05).However,therewasnosignificantdifferencebetween24and36weeksintheex

8、perimentgroups(P＞0.05).［Conclusion］Itimpliesthatinflammatarycytokinmayplayanimportantroleinthedevelopmentandprogressionofthedegenerationofcartilageendplate.　　Keywords：inflammatarycytokin;lumbarvertibrae;cartilageendplate;degenera