临床医学毕业论文il1β、il6、tnfα在兔腰椎软骨终板退变中的变化及作用.doc

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1、XX大学毕业论文IL1B、IL6、TNFa在兔腰椎软骨终板退变中的变化及作用2014年6月25日IL邛、IL6、TNFa在兔腰椎软骨终板退变中的变化及作用作者：刘斌，金大地，瞿东滨，蔡道章【摘要】［FI的］研究IL1卩、IL6、TNF(X在软骨终板退变屮的作用。［方法］新西兰白兔24只随机分为实验组与对照组。实验组通过腰椎失稳而诱导了腰椎软骨终板退变动物模型。分别于术后12、24、36周摄腰椎X线片及扫描电镜观察，检测不同吋段软骨终板屮IL1卩、IL6、TNFa含量。［结果］X线片示实验组较对照组钙化明显；Bradner指数示椎间隙高度随时间

2、延长血逐渐降低；扫描电镜示实验组软骨终板随时间延长胶原纤维逐渐变细、排列紊乱、出现裂隙，蛋白多糖丢失加剧，丄述变化较对照组显著；实验组IL1卩、IL6、TNFa含量较对照组增多(P<0.05)o［结论］IL1卩、IL6、TNFa在软骨终板退变过程屮含量和应增加，提示炎性细胞因子可能在软骨终板退变的形成和发展屮起重耍作用。【关键词】炎性细胞因子；腰椎；软骨终板；退变RoleandchangeofILip,IL6andTNFaduringrabbitlumbarcartilageendplatedegeneration//Abstract：［Ob

3、jective］Tostudytheroleofnitricoxideonthedegenerationofthecartilageendplate(CEP).［Method］TwentyfourNewZealandrabbitsweredividedintoexperimentandcontrolgroups.Intheexperimentgroup,themodeloflumbarCEPdegenerationwasestablishedbyresectionofalllumbarsupraspinousandinterspinousli

4、gaments,excisionofpartsofzygapophysialjointsanddetachmentoftheposteriorparavertebralmusclesfromlumbarvertebraes.MechanicalinstabilityofthelumbarspinecouldinducedtheprocessofCEPdegeneration.TheXrayexaminationoflumbarspinewasexaminedbyat12,24and36weekswithorwithoutopcration^r

5、cspcctivcly.ChangcsoftheultrastructurcofCEPwerealsoobservedbyscanningelectronmicroscope(SEM)duringthisprocess,andthecontentofILip,IL6,TNFaintheCEPwerealsodetectedduringtheperiods.[Result]XraygraphshowedthattheCEPcalcifiedgraduallywithtimeincreasing,andthoseoftheexperimentgr

6、oupscalcifiedmoreobviouslythanthoseofthecontrolgroups.TheSEMshowedthatcollagenfibersbecamethin,irregularandfissuredandthecontentofproteoglycandecreasedseverelywiththeelapseoftime.Thechangesinexperimentgroupsweremoreobviousthanthoseincontrolgroups.ThecontentofILip,IL6,TNFaha

7、dasignificantdifferencebetweentheexperimentgroupsandthecontrolgroups(P<0.05).However,therewasnosignificantdifferencebetween24and36weeksintheexperimentgroups(P>0.05).[Conclusion]Itimpliesthatinflammatarycytokinmayplayanimportantroleinthedevelopmentandprogressionofthedegenera

8、tionofcartilageendplate・Keywords:inflammatarycytokin;lumbarvertibrae;cartilageendp