cysteine modified gold electrode electrochemical luminescence determination of roxithromycin

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1、Cysteinemodifiedgoldelectrodeelectrochemicalluminescencedeterminationofroxithromycin[Abstract]werepreparedonbaregoldelectrodeLcysteine​​self-assembledmonolayermodifiedelectrode(LCysAu/SAM/CME).TheeffectsofrutheniumbipyridylandRoxithromycinonthismodifi

2、edelectrodeanditschemiluminescence.Theresultsshowthatthismodifiedelectrodeshowedagoodelectrochemicalactivityandelectrochemiluminescence(ECL)response.roxithromycinbasedonthepresenceofbipyridinerutheniumcanincreasetheluminousintensityforthedeterminationofLuoTablets

3、oftheelectrochemiluminescencemethod.inthebestconditions,theconcentrationofroxithromycininthe1.0×10-7~1.0×10-4mol/LanditsrelativeintensitywithinthelinearrelationshipbetweenthelineregressionequationisI=2×107C+384.02,r=0.9977;thedetectionlimit(S/N=3)is1.0×10-7mol/L.

4、Continuousdeterminationof1.8×10-5mol/Lofroxithromycin10,luminousintensityoftheRSDis1.93%,indicatingthatthismodifiedelectrodehasgoodreproducibility,andthemethodusedforthedetectionof14roxithromycintablets.[Keywords:]Lcysteine,chemicallymodifiedelectrode,roxithromyc

5、in,bipyridinerutheniumECL1IntroductionRoxithromycinas3Gerythromycinderivative(structureshowninFigure1),iserythromycinA,9-methoxy-ethoxycarbonyltooximesubstitutedasemi-syntheticerythromycin,isalargeringlactoneantibiotics[1].Theantibacterialactivityandantibacterial

6、spectrumsimilartoerythromycin,buttheacidstability,highbloodconcentration,whichhasasmalldosage,oralabsorption,theroleofalongtime,goodstability.Roxithromycinrectumforthetreatmentofchronicbacterialprostatitisandchronicnon-bacterialmajordrug[2].Atpresent,thedetermina

7、tionofroxithromycinarehighperformanceliquidchromatography[3]andflowinjectionchemiluminescence[4].ChinesePharmacopoeia(2000edition)contentwasdeterminedbymicrobiologicalassays,butthemethodiscumbersome,testinglongperiodofmanyfactors,experimentalerrorislarge.[5][6]re

8、portedthatthereferenceinChinaRoxithromycinCapsulesUSPdissolutiontestmethod.[7]TheDeterminationofRoxithromycincontent.butwiththesamesolvent,roxithromycinanditsd