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1、SensorsandActuatorsB114(2006)10011006AnovelhydrogenperoxidesensorbasedonhorseradishperoxidaseimmobilizedinDNAfilmsonagoldelectrodeYonghaiSonga,LiWangb,ChunboRena,GuoyiZhua,ZhuangLia,∗aStateKeyLaboratoryofElectroanalyticalChemistry,ChangchunInstituteofAppliedChemistry,
2、GraduateSchooloftheChineseAcademyofSciences,ChineseAcademyofSciences,RenMinStreet5625,Changchun,Jilin130022,ChinabDepartmentofAppliedChemistry,GraduateSchoolofEngineering,TohokuUniversity,Aoba-yama04,Sendai980-8579,JapanReceived15April2005;receivedinrevisedform21July
3、2005;accepted21July2005Availableonline28September2005AbstractAnovelthird-generationhydrogenperoxide(H2O2)biosensorwasdevelopedbyimmobilizinghorseradishperoxidase(HRP)onabiocompatiblegoldelectrodemodifiedwithawell-ordered,self-assembledDNAfilm.Cysteaminewasfirstself-asse
4、mbledonagoldelectrodetoprovideaninterfacefortheassemblyofDNAmolecules.ThenDNAwaschemisorbedontotheself-assembledmonolayers(SAMs)ofcysteaminetoformanetworkbycontrollingDNAconcentration.TheDNA-networkfilmobtainedprovidedabiocompatiblemicroenvironmentforenzymemolecules,g
5、reatlyamplifiedthecoverageofHRPmoleculesontheelectrodesurface,andmostimportantlycouldactasachargecarrierwhichfacilitatedtheelectrontransferbetweenHRPandtheelectrode.Finally,HRPwasadsorbedontheDNA-networkfilm.Theprocessofthebiosensorconstructionwasfollowedbyatomicforcem
6、icroscopy(AFM).Voltammetricandtime-basedamperometrictechniqueswereemployedtocharacterizethepropertiesofthebiosensorderived.Theenzymeelectrodeachieved95%ofthesteady-statecurrentwithin2sandhada0.5moll−1detectionlimitofH2O2.Furthermore,thebiosensorshowedhighsensitivity
7、,goodreproducibility,andexcellentlong-termstability.©2005ElsevierB.V.Allrightsreserved.Keywords:DNA;Horseradishperoxidase;Hydrogenperoxide;Atomicforcemicroscopy;Electrochemicalsensors1.Introductiontrodesurface,whichcanprovideapathwayofelectrontransferbetweentheredoxc
8、enterofenzymesandtheelectrodesurfaceSensitiveandaccuratedeterminationofasmallquantityof[3].Thesimplestmethodisdirectadsorptionofnat
