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1、SensorsandActuatorsB115(2006)4248Hydrogenperoxidebiosensorbasedonmyoglobin/colloidalgoldnanoparticlesimmobilizedonglassycarbonelectrodebyaNafionfilmWeiweiYang,YancaiLi,YuBai,ChangqingSun∗CollegeofChemistry,JilinUniversity,Changchun130012,PRChinaReceived26
2、April2005;receivedinrevisedform31July2005;accepted3August2005Availableonline29September2005AbstractAnovelamperometricsensorofhydrogenperoxidewasdevelopedbasedontheimmobilizationofmyoglobin(Mb)andcolloidalgoldnanopar-ticles(CGNs)onaglassycarbonelectrode(
3、GCE)byaNafionfilm.TheimmobilizedMbdisplayedapairofwell-definedandnearlyreversiblecyclicvoltammetricpeakswithaformalpotential(E◦)of−0.373Vina0.1Mphosphatebuffersolution(PBS)ofpH6.9.TheformalpotentialoftheMbhemeFe(III)/Fe(II)coupleshiftedlinearlywithpHwitha
4、slopeof−49.6mV/pH,indicatingthattheelectrontransferisaccompaniedbysingle-protontransportation.TheimmobilizedMbexhibitedexcellentelectrocatalyticresponsetothereductionofhydrogenperoxide,basedonwhichanunmediatedbiosensorforhydrogenperoxidewasachieved.Thel
5、inearrangefordeterminationofhydrogenperoxidewasfrom1.5×10−6to9.0×10−5Mwithadetectionlimitof5.0×10−7Matasignal-to-noiseratioof3.Thestability,repeatabilityandselectivityofthebiosensorwerealsoevaluated.©2005ElsevierB.V.Allrightsreserved.Keywords:Myoglobin;
6、Colloidalgoldnanoparticles;Directelectrochemistry;H2O2biosensor;Nafion1.IntroductionimmobilizedHRP[1014].ButitisstillnecessarytofindothersubstitutesforHRPtofabricatenovelH2O2biosensors.Myo-Theaccuratedeterminationofhydrogenperoxide(H2O2)globin(Mb)isknownt
7、ohavesomeintrinsicperoxidaseactivityplaysanimportantroleinmanyfieldsincludingindustry,clini-duetoitsclosesimilaritytoperoxidase.Therefore,itmightbecalcontrolandenvironmentalprotection[13].ManytraditionalpossibletoemployMbcontainingahemegroupthatservesast
8、echniquessuchastitrimetry[4],spectrophotometry[5],chemi-theactivecentertocatalyzethereductionofH2O2.However,luminescence[6]havebeenemployedforthispurpose.How-electrontransferbetweenMbandbaresolidelectrodesisusu-ever,manyofthesepr
