资源描述：
《8-氯腺苷酸诱导多发性骨髓瘤细胞凋亡的实验研究》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。
1、8-氯腺苷酸诱导多发性骨髓瘤细胞凋亡的实验研究?1204?中国实验血液学杂志JournalofExperimentalHematology2007;15(6):1204—1207文章编号(ArticleID):1009-2137(2007)06—1204—04?论着?8-氯腺苷酸诱导多发性骨髓瘤细胞凋亡的实验研究程毅敏,朱琦,贾培敏,胡钧培上海交通大学医学院第九人民医院血液科,上海200011;上海血液学研究所,上海200052摘要本研究探究8一氯腺苷酸对多发性骨髓瘤细胞的可能作用.以多发性骨髓瘤细胞株RPMI8226细胞为体外模型,在观察8一氯腺苷酸对细胞
2、生长,活力及形态学影响的基础上,应用流式细胞术检测细胞DNA含量分布和细胞表面Annexin—V的含量,以DNA凝胶电泳技术评判细胞凋亡与否,并应用Westernblot检测药物处理前后细胞内细胞周期调控蛋白cylinE和CDK2的变化.结果表明:低浓度8一氯腺苷酸(1—30p~nol/L)能够明显地抑制RPMI8226细胞的生长,显着降低其细胞活力,且呈时间和浓度依赖性.进一步细胞形态学观察,流式细胞术及DNA凝胶电泳分析显示,这些浓度的8?氯腺苷酸诱导RPMI8226细胞凋亡.Westernblot检测表明,8一氯腺苷酸可通过影响细胞周期调控蛋白cyli
3、nE和CDK2的表达而干扰细胞增殖.结论:8一氯腺苷酸能够有效地抑制多发性骨髓瘤细胞生长并诱导其凋亡.关键词8一氯腺苷酸;多发性骨髓瘤;细胞凋亡中图分类号R733,3;R979.1文献标识码AApoptosis0fMultipleMyelomaCellsInducedby8-CI-CAMPVitroCHENGYi.Min.ZHUaf,JIAPei.n,HUJun—PeiDepartmentofHematology,NinthPeopleHospital,ShanghaiJiaotongUniversityMedicalCollege,Shanghai2000
4、11,China;'ShanghaiInstituteofHematology,Shanghai200025,ChinaAbstractThestudywasaimedtoinvestigatethepossibleeffectsof8-chloroadenosine35"-monophosphate(8-Cl—cAMP)onthemultiplemyelomacells.ThemultiplemyelomacelllineRPM18226wasusedasinvitromodels.TheeffectongrowthinhibitionofRPMI8226
5、cellsWasevaluatedbycellgrowthandviabilitycurve,DNAfragmentwasvisu—alizedbyagarosegelelectrophoresis.111eamountofapoptosiscellsWasmeasuredbyflowcytometry.MeanwhileWest-erablotassaywereusedtodetectthechangeofseveralkeycellcycleregulatoryproteinsCDK2andcyclinEinthesecellsbeforeandafte
6、rthetreatment.TheresultsshowedthatlOWdose8-Cl—cAMP(1—30l~nol/L)inhibitedtheproli—ferationandviabilityofRPMI8226cellssignificantly.AgarosegelelectrophoresisofDNArevealedtheapoptosischarac—teristic"ladder"pmtem.ApoptosiswasalSOconfirmedbyflowc~ometry.Inaddition.8-Cl—cAMPwasabletoinhi
7、bitthecellgrowththroughmodulatingexpressionofcellcycleregulatorsCDK2andcyclinE.Itisconcludedthat8-cl—cAMPinhibitstheproliferationandinduceapoptosisofmultiplemyelomacellseffectively.Keywords8-chloroadenosine3,5一monophosphate;multiplemyeloma;apoptosisJExpHematof2007;15(6):1204—1207多发
8、性骨髓瘤(multiplemyeloma,MM)是一
