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1、壳聚糖和尿囊素预防外科术后腹腔粘连效果的观察论文沈新，李孟彬，王为忠，施海【关键词】粘连；壳聚糖；尿囊素；兔【Abstract】AIM:Toinvestigatethepreventiveeffectofchitosanandallantoinagainstpostsurgicalabdominaladhesionsandtostudythesynergeticeffectsofthesetechanism.METHODS:Niysixrabbitsodels,lyandevenlydividedintocontrolgroup(groupA)，chitosantreatmentgrou
2、p(groupB),allantointreatmentgroup(groupC)andchitosan+allantointreatmentgroup(groupD).Rightaftertheoperation,thesituationsofrabbitsicroscope.RESULTS:Themodelsofadhesionsmationreactionandthedegreeoffibrosisparedentinaladhesions,andthetol/L浓度的溶液备用；速眠新Ⅱ注射液，1.5mL/支，长春军需大学兽医研究所生产.1.2方法1.2.1动物准备全部动物术前均禁
3、食(不禁水)12h，速眠新Ⅱ注射液（2mL/kg）肌肉注射麻醉.麻醉后仰卧位固定，剪毛消毒铺单，无菌操作下取腹正中切口，长约4cm.打开腹腔后找出盲肠，用组织钳取出盲肠，用显微尖镊于肠系膜对侧沿纵轴方向剥离约5cm×2cm大小浆膜［3］.1.2.2实验动物分组家兔按双盲法随机分为4组，每组24只：对照组(A组)，关腹前用生理盐水30mL冲洗腹腔，吸弃部分液体，将盲肠放回腹腔，关腹；壳聚糖组(B组)，关腹前用壳聚糖凝胶均匀涂布于距盲端3cm范围内的盲肠浆膜面(用量：1mL/只)；尿囊素组(C组)关腹前注入尿囊素溶液30mL；壳聚糖+尿囊素联合用药组(D组)，关腹前均匀涂布壳聚糖凝胶(1mL
4、/只)并注入尿囊素溶液30mL.1.2.3目视判断评分术后动物禁食12h，分笼饲养，饲料为全价营养家兔颗粒饲料.每组再随机分为2组，每组12只，分别于术后1enyi检验.2结果2.1一般情况各组家兔麻醉清醒后即可活动，精神、进食良好，伤口愈合好.术后4d，A组动物死亡1只，剖腹探查死因为广泛肠粘连致绞窄性肠梗阻.术后1akosN,FinePM,etal.Peritonealadhesions:etiology,.freelanagement［J］.DigSurg,2001,18(4):260-273.［2］RasaK,ErverdiN,KarabulutZ,etal.Theeffecto
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6、ventionofintraabdominaladhesions［J］.AmJSurg,1993,165(2):229-232.［5］KramerK,SenningerN,HerbstH,etal.Effectivepreventionofadhesionsation［J］.EurSurgRes,2005,37(4):235-241.［7］GorvyDA,HerrickSE,ShahM,etal.Experimentalmanipulationoftransforminggrossignificantlyaffectsadhesionformationinamurinesurgicalm
7、odel［J］.AmJPathol,2005,167(4):1005-1019.［8］KojimaK,OkamotoY,KojimaK,etal.Effectsofchitinandchitosanoncollagensynthesisinpaireddb/dbmiceuraY,NomuraA,MinamiS,etal.Effectsofchitin/chitosanandtheiroligomers/monomersonrelea
