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1、中国水稻科学(ChinJRiceSci),２０１６,３０(４):３４５－３５５http://www．ricesci．cnDOI:１０．１６８１９/j．１００１Ｇ７２１６．２０１６．５１８４３４５水稻脆秆突变体bc１６的鉴定和基因精细定位舒亚洲曾冬冬秦冉金晓丽郑希石春海∗(浙江大学农业与生物技术学院农学系,杭州３１００５８;∗通讯联系人,EＧmail:chhshi＠zju．edu．cn)IdentificationandGeneFineMappingofaBrittleCulm１６(bc１６)MutantinRiceSHUYaＧzhou,ZENGDongＧd
2、ong,QINRan,JINXiaoＧli,ZHENGXi,SHIChunＧhai∗(CollegeofAgricultureandBiotechnology,ZhejiangUniversity,Hangzhou３１００５８,China;∗Correspondingauthor,EＧmail:chhshi＠zjue．duc．n)SHUYazhou,ZENGDongdong,QINRan,etal．Identificationandgenefinemappingofabrittleculm１６(bc１６)muＧtantinrice．ChinJRiceSci
3、,２０１６,３０(４):３４５Ｇ３５５．Abstract:Brittleculmmutantsareoneoftheimportantmaterialstostudyplantmechanicalstrength．Abrittleculmmutantnamedasbc１６(brittleculm１６)wasisolatedfromNipponbarethroughEMS(EthylMethaneSulphonate)treatment．Comparedwithwildtype,bc１６featuredfragileandeasilybrokenstems,
4、leaves,roots,lowerplantheightandfilledgrainsperpanicle,shorterpaniclelengthandmainrootlength．Thecomponentsanalysisofstemcellwallshowedthatthecellulosecontentinbc１６mutantwassharplydecreased,whilehemiＧcellulosecontentwasevidentlyinＧcreasedandnosignificantdifferencehadbeenfoundinthel
5、ignincontentbetweenbc１６mutantandwildtype．Thecellshapeandarrangementofparenchymacellsinbc１６mutantbecameirregularanddisordered．Moreover,theparenchymacellwallsandthesecondarycellwallsofsclerenchymacellsinbc１６mutantundertheepidermislayerwerethinnerthanthoseinwildtype．Theresultsofgenet
6、icanalysisrevealedthatthemutanttraitwascontrolledbyasinglerecessivegene,whichwasmappedina６６．６ＧkbregionbetweenInDel(insertionＧdeletion)markers２ＧFand２ＧHonchromosome２with７candidategenes．Amongthesegenes,Os０２g０７３８９００,whichisallelictoBC３gene,wasspeciallyfocusedon．thTherewasaTtoAsubstitu
7、tionattheveryendofthe１３thintronofOs０２g０７３８９００whichwasatthe５１１３baselocaＧtionfrominitiationcodonbysequencing,whichledansplicingforwardmutationintheprocessoftranscription,andsixnucleotideswerecutintomRNAasaresult,whichfinallycausethestopoftranslation．Additionally,inthemutant,therewer
8、esignificantdecreaseintheexpressi
