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1、中国水稻科学(ChinJRiceSci),２０１５,２９(６):５８７－５９４http://www．ricesci．cnDOI:１０．３９６９/ji．ssn．１００１Ｇ７２１６．２０１５．０６．００４５８７水稻窄叶突变体nal１０的鉴定与基因精细定位方云霞１,２,３,＃朱丽２,＃潘江杰４余海平２薛大伟４饶玉春２王小虎２石珍源２张栋４祝阳舟２董国军２曾大力２张光恒２郭龙彪２胡江２,∗钱前２,∗(１浙江大学农业与生物技术学院,杭州３１００５８;２中国水稻研究所水稻生物学国家重点实验室,杭州３１０００６;３江西省农业科学院
2、水稻研究所,南昌３３０２００;４杭州师范大学生命与环境学院,杭州３１００３６;＃共同第一作者;∗通讯联系人,EＧmail:qianqian１８８＠hotmail．com;hujiang５８８＠１６３．com)IdentificationandFineMappingofaNarrowLeafMutantnal１０inRiceFANGYunＧxia１,２,３,＃,ZHULi１,＃,PANJiangＧjie４,YUHaiＧping２,XUEDaＧwei４,RAOYuＧchun２,WANGXiaoＧhu２,SHIZhenＧy
3、uan２,ZHANGDong４,ZHUyangＧzhou２,DONGGuoＧjun２,ZENGDaＧli２,ZHANGGuangＧheng２,GUOLongＧbiao２,HUJiang２,∗,QIANQian２,∗(１CollegeofAgricultureandBiotechnology,ZhejiangUniversity,Hangzhou３１００５８,China;２StateKeyLaboratoryofRiceBiology,ChinaNationalRiceResearchInstitute,Hangzho
4、u３１０００６,China;３RiceResearchInstitute,JiangxiAcademyofAgriculturalSciences,Nanchang３３０２００,China;４CollegeofLifeandEnvironmentalSciences,HangzhouNormalUniversity,Hangzhou３１００３６,China;＃Theseauthorscontributedequallytothiswork;∗Correspondingauthors,EＧmail:qianqian１８
5、８＠hotmailc．om;hujiang５８８＠１６３c．om)FANGYunxia,ZHULi,PANJiangjie,etal．Identificationandfinemappingofanarrowleafmutantnal１０inrice．ChinJRiceSci,２０１５,２９(６):５８７Ｇ５９４．Abstract:Anovelricemutantnarrowleaf１０(nal１０)wasidentifiedfromanEMS(ethylmethanesulphonate)mutagenizedpo
6、pulationofWuyunjing７．Themutantwascharacterizedbynarrowleavesandsmallerarchitecturephenotypeattheseedlingstage,anddwarf,narrowleaves,deformedflowersandmalesterilityatthereproductionstage．CytoＧanatomicalanalysisindicatedthatthemutanthadfewerlargeandsmallveinscomp
7、aredwiththewildＧtype,whichmayaccountforthenarrowleafphenotype．Thegeneticanalysisindicatedthenarrowleaftraitwascontrolledbyarecessivenucleargene．UsingF２populationderivedfromthecrossofnal１０andTaizhongbendi１,threecloselylinkedSSRmarkersRM１２８７,RM５６２andRM５６３８onchrom
8、osome１wereobtainedbythebulkedsegregantanalysismethod．Withthedevelopednewmarkers,thegenewasfinallyfineＧmappedtoanintervalof５５．２kbbetweentheSTSmarkersPK８３andPK７８,whereatotalof
