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1、中国水稻科学(ChinJRiceSci),２０１５,２９(６):５９５－６００http://www．ricesci．cnDOI:１０．３９６９/ji．ssn．１００１Ｇ７２１６．２０１５．０６．００５５９５水稻窄卷叶突变体Nrl３(t)的基因定位张小惠１,２,３,＃秦亚芝１,２,＃张迎信２占小登２张振华２沈希宏２程式华２曹立勇２,∗吴先军１,∗(１四川农业大学水稻研究所/西南作物基因资源与遗传改良教育部重点实验室,成都６１１１３０;２中国水稻研究所国家水稻改良中心,杭州３１０００６;３浙江国稻高科技种业有限公司杭州
2、３１１４０１;＃共同第一作者;∗通讯联系人,EＧmail:wuxjsau＠１２６．com;caolycgf＠mail．hz．zj．cn)GeneMappingofaNarrowandRolledLeafMutantNrl３(t)inRiceZHANGXiaoＧhui１,２,３,＃,QINYaＧzhi１,２,＃,ZHANGYingＧxin２,ZHANXiaoＧdeng２,ZHANGZhenＧhua２,SHENXiＧhong２,CHENGShiＧhua２,CAOLiＧyong２,∗,WUXianＧjun１,∗(１Ric
3、eResearchInstitute/KeyLaboratoryofSouthwestCropGeneticResourcesandImprovement,MinistryofEducation,SichuanAgriculturalUniversity,Chengdu６１１１３０,China;２NationalCenterforRiceImprovement,ChinaNationalRiceResearchInstitute,Hangzhou,３１０００６,China;３ZhejiangNationalRice
4、HighＧTechPlantsIndustryLimitedCompany,Hangzhou３１１４０１,China;＃Theseauthorscontributedequallytothiswork;∗Correspondingauthors,EＧmail:wuxjsau＠１２６c．om;caolycgf＠mail．hz．zjc．n)ZHANGXiaohui,QINYazhi,ZHANGYingxin,etal．GenemappingofanarrowandrolledleafmutantNrl３(t)inric
5、e．ChinJRiceSci,２０１５,２９(６):５９５Ｇ６００．Abstract:Nrl３(t)wasanovelnarrowandrolledleafmutantfromtheindicarestorerlineZhonghui８０１５afterexposure６０toCoＧγray．Nrl３(t)exhibitedsignificantadaxiallyＧrolledleaves,reducedplantheight,shortenedpaniclelength,reducedgrainlength,gra
6、inwidthandthousandＧgrainweight,aswellastheseedＧsettingrate．Cytologicalanalysisshowedthattherolledleafphenotypewasduetothereducednumberofvascularbundleandthechangedsizeofbulliformcells．InaninitialmappingwithF２individualsderivedfromacrossbetweenNrl３(t)andjaponic
7、a０２４２８,thenarrowandrolledleafgeneNrl３(t)waseventuallymappedtoa７０．３kbregiononthelongarmofchromosome２betweentwonewlydevelopedsequencetaggedsitemarkersC９andC１０E．TheseresultswouldlayagoodfoundationformolecularcloningandfunctionalanalysisofNrl３(t)．Keywords:rice;nar
8、rowandrolledleaf;gene;finemapping张小惠,秦亚芝,张迎信,等．水稻窄卷叶突变体Nrl３(t)的基因定位．中国水稻科学,２０１５,２９(６):５９５Ｇ６００．摘要:利用６０CoＧγ射线辐射诱变籼稻恢复系中恢８０１５,获得了一份窄卷叶突变体Nrl３(t).与野生型中恢８０１５相比,突变体叶片显著变窄内卷、株高降低、穗长变短、结实率
