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1、作物学报ACTAAGRONOMICASINICA2016,42(7):966975http://zwxb.chinacrops.org/ISSN0496-3490;CODENTSHPA9E-mail:xbzw@chinajournal.net.cnDOI:10.3724/SP.J.1006.2016.00966一个水稻显性斑点叶突变体的鉴定和基因精细定位郭丹施勇烽王惠梅张晓波宋莉欣徐霞贺彦*郭梁吴建利中国水稻研究所/水稻生物学国家重点实验室/国家水稻改良中心,浙江杭州310006摘要:通过EMS(ethanemethyls
2、ulfonate)诱变籼稻品种IR64获得一个稳定遗传的显性斑点叶突变体HM113。在大田环境下,突变体褐色斑点在播种后3周的叶片上产生,始穗期扩散至叶鞘。与野生型IR64相比,突变体HM113的株高、结实率和千粒重等农艺性状显著下降,光合色素含量、净光合速率和可溶性蛋白含量显著降低。同时突变体CAT和SOD活性显著降低,POD活性显著上升。组织化学分析显示,突变体叶片中积累了大量活性氧,且斑点处细胞坏死。白叶枯病菌接种结果显示,HM113是一个广谱抗性增强的突变体。实时定量PCR分析表明HM113中防卫反HM113应基因A
3、OS2、PAL4、PR10和PR1b等的表达大幅上调。遗传分析表明,突变体褐斑性状受单显性基因(Spl)控制,利用图位克隆法将该基因定位在第7染色体长臂RM21605和RM418之间,物理距离约为308kb。本研究为褐HM113斑基因Spl的克隆与功能分析奠定了基础。关键词:水稻;斑点叶突变体;白叶枯病抗性;活性氧;基因定位CharacterizationandGeneFineMappingofaRiceDominantSpotted-leafMutantGUODan,SHIYong-Feng,WANGHui-Mei,ZHA
4、NGXiao-Bo,SONGLi-Xin,XUXia,HEYan,GUO*Liang,andWUJian-LiStateKeyLaboratoryofRiceBiology/ChineseNationalCenterforRiceImprovement/ChinaNationalRiceResearchInstitute,Hangzhou310006,ChinaAbstract:Astableinheritedricespotted-leafmutantHM113wasisolatedfromanEMS-inducedIR6
5、4mutantbank.Undernaturalconditions,brownlesionswereobservedontheleavesinthreeweeksaftersowingandspreadtothesheathsattheinitialheadingstage.Agronomictraitsincludingtheplantheight,paniclelength,numberofpanicles,numberoffilledgrain/panicle,seed-settingrateand1000-grai
6、nweightweredecreasedsignificantlyinHM113.Inaddition,thephotosyntheticpigmentcontents,netphoto-syntheticrateandsolubleproteincontentinthemutantweresignificantlylowerthanthoseinthewildtypeIR64,whiletheMDAcontentwassimilartothatinthewild-type.ActivitiesofCATandSODwere
7、significantlylowerandactivityofPODwassignifi-cantlyhigherinthemutantthaninIR64.HistochemicalanalysisshowedthatcelldeathandROSaccumulationwereoccurredinandaroundthelesionsinHM113.Furthermore,diseaseresistancetobacterialblightpathogenswassignificantlyenhancedinthemut
8、antincontrasttothatinthewildtypeIR64.Expressionofdefense-relatedgenesincludingAOS2,PAL4,PR10,andPR1bwasapparentlyup-regulatedinthemutant.Genetica
