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《水稻显性脆秆突变体Bc18的鉴定和基因定位》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。
1、中国水稻科学(ChinJRiceSci),２０１６,３０(２):１２７－１３５http://www．ricesci．cnDOI:１０．１６８１９/j．１００１Ｇ７２１６．２０１６．５１６２１２７水稻显性脆秆突变体Bc１８的鉴定和基因定位彭应财１,２,＃刘文真２,＃傅亚萍２王鹤潼１胡国成２陈温福１徐正进１,∗(１沈阳农业大学农学院,沈阳１１０８６６;２中国水稻研究所,杭州３１０００６;＃共同第一作者;∗通讯联系人,EＧmail:xuzhengjin＠１２６．com)CharacterizationandGeneMappingofaDo
2、minantBrittleCulmMutantBc１８inRice(OryzasativaL．)PENGYingＧcai１,２,＃,LIUWenＧzhen２,＃,FUYaＧping２,WANGHeＧtong１,HUGuoＧcheng２,CHENWenＧfu１,XUZhengＧjin１,∗(１CollegeofAgronomy,ShenyangAgriculturalUniversity,Shenyang１１０８６６,China;２ChinaNationalRiceResearchInstitue,Hangzhou３１０００６,Chi
3、na;＃Theseauthorscontributedequallytothiswork;∗Correspondingauthor,EＧmail:xuzhengjin＠１２６．com)PENGYingcai,LIUWenzhen,FUYaping,etal．CharacterizationandgenemappingofadominantbrittleculmmutantBc１８inrice(OryzasativaL．)．ChinJRiceSci,２０１６,３０(２):１２７Ｇ１３５．Abstract:Abrittleculmmut
4、antwasobtainedfromtheF２populationofthreeＧwaycrossⅡＧ３２B//XieqingzaoB/DularandnamedasBrittleculm１８(Bc１８)accordingtoitsphenotypes．Eachpartofplantshowedbrittlenessduringthewholegrowthperiod．Inordertoidentifythemutant,nearisogeniclinepopulationsofZhongcuiBandZhong９Bwerecrea
5、tedwithBc１８mutantasthedonorofbrittlegeneandZhong９B,anormalculmＧstrengthvariety,asthereceptorandrecurrentparent．Comparedwithwildtype,Bc１８significantlydeclinedby７０．７０％and４７．１６％inmechanicalstrengthofculmandleaves,respectively．Nosignificantdifferenceswerefoundintermsofgrow
6、thduration,plantheight,paniclenumberperplant,spikeletnumberperpanicle,seedsettingrateand１０００Ｇgrainweight．CellwallcomponentsanalysesshowedthatcellulosecontentandlignincontentinBc１８hadnosignificantdifferenceascomparedwiththoseofwildtype,whilehemicellulosecontentinculmand
7、leafdramaticallyincreasedby３１．８４％and１７．３５％,respectively．GeneticanalysesofsixF２andtwelveBC１F１backcrosspopulationsrevealedthatthephenotypeofBc１８wascontrolledbyasingledominantgene．Bc１８/０２４２８andBc１８/９３１１F２populationsweredevelopedforBc１８genemapping．BymeansofmapＧbasedcloning
8、technology,withsomeSSRmarkerspublishedonlineandnewdesignedInDelmarkers,Bc１８waslocalizedbetweenInDelmarkerPBC２２andPBC３
