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1、中国水稻科学(ChinJRiceSci),2013,27(3):240-246http://www.ricesci.cn240DOI:10.3969/j.issn.1001-7216.2013.03.003水稻白化致死突变体abl4的鉴定和基因定位程世超1,2刘合芹2翟国伟2冯世座1,2赵辉1,2汪得凯2,*陶跃之2(1浙江师范大学化学与生命科学院,浙江金华321004;2浙江省农业科学院作物与核技术利用研究所,浙江杭州310021;*通讯联系人,E-mail:310021kay77@163.com)GeneticAnalysisandGeneMappingo
2、fanalbinolethalMutantinRiceCHENGShi-chao1,2,LIUHe-qin2,ZHAIGuo-wei2,FENGShi-zuo1,2,ZHAOHui1,2,WANGDe-kai2,*,TAOYue-zhi2(1CollegeofChemistryandLifeSciences,ZhejiangNormalUniversity,Jinhua321004,China;2InstituteofCropandNuclearTechnologyUtilization,ZhejiangAcademyofAgriculturalScience
3、s,Hangzhou310021,China;*Correspondingauthor,E-mail:310021kay77@163.com)CHENGShichao,LIUHeqin,ZHAIGuowei,etal.Geneticanalysisandgenemappingofanalbinolethalmutantinrice.ChinJRiceSci,2013,27(3):240-246.60Abstract:Analbinolethalmutant,temporarilydesignatedasalbinolethal4(abl4),wasobtain
4、edfromCoγ-rayradiationmutantpoolofjaponicariceZhonghua11.Theseedlingofabl4showedalbinophenotypefromgerminationto3-leaf-stage,thendied.Thechlorophyllandcarotenoidcontentsinabl4mutantweretoolowtobedetected.Moreover,theelectrontransportrateandtheeffectivequantumyieldofphotosystemⅡinabl
5、4wereaboutzero.Ontheotherhand,thepotentialphotochemicalefficiencyofPSⅡinabl4wasalsoatanundetectablylowlevel,indicatingthattheactivityofphotochemicalreactionwaslostintheleavesofabl4.Theactivitiesofanti-oxidantenzymesindudingsuperoxidedismutaseandperoxidaseweresignificantlyincreased,w
6、hilecatalasewasdecreasedsignificantly.Asresults,thecontentofmalondialdehydewasalsoincreasedsignificantlyinabl4comparedwiththewildtype,suggestingthatabl4wasstronglysubjectedtohighoxidantstress.Transmissionelectronmicroscopyexaminationshowedthatabl4plastidscontainedsomeemptyvesicleswi
7、thoutthylakoids.Itwasrevealedthatthephenotypeofabl4wascontrolledbyasinglerecessivenucleargene.AnF2populationgeneratedbycrossingtheabl4mutantwiththeindicaricecultivarLongtefuBwasusedforgenemapping,ABL4waspreliminarilylocatedbetweenRM3785andRM303onricechromosome4andfurthermappedtoa201
8、kbregion.Keywords:r
