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1、作物学报ACTAAGRONOMICASINICA2014,40(7):1182−1189http://zwxb.chinacrops.org/ISSN0496-3490;CODENTSHPA9E-mail:xbzw@chinajournal.net.cnDOI:10.3724/SP.J.1006.2014.01182水稻早衰突变体esl5的鉴定及其基因精细定位*桑贤春徐芳芳朱小燕邢亚迪何沛龙张长伟杨正林何光华西南大学水稻研究所/转基因植物与安全控制重庆市重点实验室/南方山地农业教育部工程研究中心,重庆400716摘要:叶片早衰直接影响作物产量
2、和品质,鉴定早衰突变体、图位克隆调控基因对于研究植物衰老机制具有重要的意义。以甲基磺酸乙酯诱变水稻籼型恢复系缙恢10号,获得一个早衰突变体esl5(earlysenescentleafmutant5),本文对其进行了形态鉴定、细胞学观察、理化分析和基因定位等研究。结果表明,与野生型相比,esl5的苗期叶片正常,分蘖期呈黄绿色,孕穗期开始叶片中上部逐渐黄化衰老;衰老部位的细胞结构异常,细胞膜降解,叶绿体基质片层疏松、排列不规则,光合色素含量和光合速率极显著下降。此外,esl5的·OH和H2O2含量极显著升高,SOD和CAT的活性则极显著降低。与
3、野生型相比,esl5的生育期延长20d左右,千粒重显著增加,穗粒数、实粒数和结实率则显著降低。esl5突变性状受1对隐性核基因调控,精细定位在第3染色体Indel标记Indel03-1和Indel03-2之间83.4kb的物理范围内,包含11个注释基因,这为ESL5的克隆和功能研究奠定了基础,也有利于水稻品种的遗传改良。关键词:水稻(Oryzasativa);早衰;基因定位;活性氧(ROS);光系统IdentificationandGeneFineMappingofEarlySenescentLeafMutantesl5inOryzasati
4、vaSANGXian-Chun,XUFang-Fang,ZHUXiao-Yan,XINGYa-Di,HEPei-Long,ZHANGChang-Wei,YANG*Zheng-Lin,andHEGuang-HuaRiceResearchInstituteofSouthwestUniversity/ChongqingKeyLaboratoryofApplicationandSafetyControlofGeneticallyModifiedCrops/EngineeringResearchCenterofSouthUplandAgricultur
5、e,MinistryofEducation,Chongqing400716,ChinaAbstract:Prematuresenilitydirectlyinfluencescropyieldandqualityintheproduction.Therefore,itiscrucialtoidentifyearlysenescentleafmutantsandthenclonegenesassociatedwithsenescencebymap-basedstrategy,whichissignificantintheresearchofse
6、nescencemechanism.Anearlysenescentleafmutantesl5hasbeendiscoveredfromtheprogenyofindicarestorerlineJin-hui10withseedstreatedbyethylmethanesulfonate,thispaperperformedthestudiesonitsmorphologicalidentification,cyto-logicalobservation,physiologicalanalysisandgenemapping.There
7、sultsshowedthattheesl5demonstratednormalphenotypeattheseedlingstage,displayedyellowgreencoloratthetilleringstage,andappearedyellowandsenescentleafbladesatbootingstage.Cellstructureoftheesl5wasbadlyinfluenced,showingdestroyedcellmembranes,looseandirregularstromalamellainthes
8、ectionofsenescentleaves.Chlorophyllcontentandphotosyntheticrateoftheesl5weresignif
