粗叶悬钩子总生物碱对非酒精性脂肪肝大鼠脂代谢及CPTⅠ的影响.pdf

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1、第27卷第6期长治医学院学报Vo1．27NO．62013拒12月JOURNALOFCHANGZHIMEDICALCOLLEGEDec．2013417粗叶悬钩子总生物碱对非酒精性脂肪肝大鼠脂代谢及CPT工的影响伍娟钟晓勇赵锦燕郑玉清郑海音洪振丰摘要目的：探讨粗叶悬钩子总生物碱(TARAP)对非酒精性脂肪肝(NAFLD)大鼠脂代谢及肝脏形态学的影响，研究其作用机制。方法：购入的SD大鼠适应性喂养7d，然后按照体重分层随机分为正常组、模型组、东宝肝泰组、TARAP低剂量组和高剂量组，正常组饲以基础饲料，饮用自来水，其余各组饲以高脂饲料，持续喂养12周。造模成功后各组予以相应药

2、物灌胃4周后取材，HE染色，观察肝组织病理形态学变化。RT—PCR和免疫组化法检测肉碱脂酰转移酶I(carnitinepalmityltransferase工，CPTI)的基因和蛋白表达。结果：TARAP低剂量组、高剂量组脂肪变细胞较模型组明显减少；RT-PCR和免疫组化结果显示，TARAP能显著提高CPTI的表达。结论：TARAP能提高CPTI的表达，减轻肝细胞脂肪变性，提示TARAP通过调节CPTI的表达是其治疗NAFLD作用机制之一。关键词粗叶悬钩子总生物碱；非酒精性脂肪肝；CPTI中图分类号R285．5文献标志码A文献编号1006一(2013)06—417—04

3、EfectsofTotalAlkaloidsofRubusalcmefoiinsFironOillipidmetabolismandCPT[Expr~oninRatswithNAFLDWuJuan，ZhongXiaoyong，ZhaoJinyan，etalUniversityoftraditionalChineseMedicineAbstract0bjective：ToinvestigatetheeffectsandmechanismofTotalAlkaloidsofRubusalceaefoliusPiron(TARAP)onlipidmetabolismandth

4、elivermorphologyinratswithnonalcoholicfattyliverdesease(NAFLD)，andtoexploretheinmolvedmechanism．Methods：SDratswererandomlydividedintonormalgroup，modelgroup，DongbaoGantaigroup，TARAPlowdosegroupandhighdosegroupaccordingtOthebodyweight．Normalgroupwasfedwithbasicdietandwater，othergroupsweref

5、edwithhighfatdietfor12weeksconstantly．AftertheestablishmentofNAFLDratsmodel，eachgroupsweregivencorrespondingmedicinetreatmentorinterference．After4weeksinterference，livertissueofeachratswereobtainedandHEstainingwasperformedtOobservethepathologicalchangesoflivertissue．RT—PCRandimmunohistoc

6、hemistryanalysiswereusedtodetectedthegeneandproteinexpressionofCPTIinrats’livertissue．Results：LivercellswithfattydegenerationweresignificantlydecreasedinTARAPlOWdosegroupandhighdosegroupthanthatinmodelgroup；RT-PCRandimmunohistochemistryresultsshowedthat，TARAPcouldsignificantlyincreasethe

7、geneandproteinexpressionofCPTI．Conclusion：TARAPcanobviouslyattenuatefattydegenerationinlivercellsofNAFLDrats，whichmightprobablyregulatedbytheexpressionofCPTI．KeywordsTARAP；NAFLD；CPTI非酒精性脂肪肝(nonalcoholicfatty炎、止血和促进凝血、抗氧化等作用口]。课题组前liverdisease，NAFLD)已成为西方国家肝脏转氨酶期研究结果表明，粗叶悬