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时间:2020-05-24
《牡蛎蛋白酶解物改善小鼠学习记忆能力及其体内抗氧化活性的研究.pdf》由会员上传分享,免费在线阅读,更多相关内容在应用文档-天天文库。
1、食品科技2o13年第38卷第O蝴FOODSC-ENCEANDTECHNoLoGY目品开发牡蛎蛋白酶解物改善▲鼠学习记雒力及其体内抗氧=jl活性的研究林海生,曹文红r,卢虹玉,章超桦t,秦小明,3谢敏(1.广东海洋大学食品科技学院,湛江524088:2.广东省水产品深加工广东普通高校重点实验室,湛江524088;3.国家贝类加工技术研发分中心,湛江524088)摘要:探讨牡蛎蛋白酶解物(Enzymatichydrolysatesfromoysterprotein,以下简称EHOP)改善小鼠学习记忆能力及其体内抗氧化活性。选用中性蛋白酶和碱性蛋白酶分别水解牡蛎蛋白得到EHOP1和E
2、HOP2,按剂量5g/kg·d分别给4周龄小鼠灌胃4周后,通过Morris水迷宫方法考察了EHOP对小鼠空间学习记忆功能的影响,并检测小鼠肝组织和脑组织中SOD活性及MDA含量。结果表明,与空白组相比,EHOP1组和EHOP2组小鼠的潜伏期(分别为18.97±3.84S、22.61±4.04s)均明显缩短,采用空间搜索策略的比例、穿越平台的次数、目标象限停留的时间和路程均有不同程度的增加。与空白组相比,EHOP能够显著增强小鼠肝组织中sOD活性(P<0.005),显著性减少脑组织及血浆中的MDA含量(P3、有良好的体内抗氧化活性。关键词:酶解物;Morris水迷宫;学习记忆;抗氧化中图分类号:TS201.4文献标志码:A文章编号:1005—9989(2013)04—0037—05ImprovingeffectofenzymatichydrolysatesfrOmoysterproteinontheabilityoflearningmemoryinmiceandtheirantioxidantactivitiesinvivoLINHai—sheng,CAOWen—hong',LUHong-yu,ZHANGChao—hua,,,QINXiao—ming,,XIEMin(1.FoodS4、cienceandTechnologyInstitute,GuangdongOceanUniversity,Zhanjiang524088;2.KeyLaboratoryofAquaticProductAdvancedProcessingofGuangdongHigherEducationInstitutes,Zhanjiang524088;3.NationalR&DBranchCenterforShellfishProcessing,Zhanjiang524088)Abstract:Toinvestigatetheeffectsofenzymatichydrolysatesf5、romoysterprotein(EHOPforshort)ontheabilityoflearningmemoryinmiceandtheirantioxidantactivities.EHOP1(usingneutralprotease)andEHOP2(usingalkalineprotease)wereseparatelyappliedto4week—oldmiceatadoesof5.0g/kg‘dfor4weeksandthelearningandmemoryabilitywasdeterminedusingtheMorriswatermazetests(MWM)w6、ithTaurine(Tau)ascontrolgroupandnormalsaline(NS)group.Inordertoinvestigatetheantioxidant收稿日期:2012—11-09通讯作者基金项目:国家现代农业产业技术体系项目(CARS一48;贝类)。作者简介:林海生(1985-),男,硕士研究生。。i食品科技FOODSCIENCEANDTECHNOLOGY2013~第38卷第O4期activitieslnvivo.theSODactivityandMDAcontentinbrainandIiverwereassayed.TheMWMshowedtha7、tEHOP1andEHOP2groupsledtoareductioninescapeIatencyf18.97+3.84Sand22.61±4.04S1comparedwiththeNSgroup.Andtheproportionofspatialstrategy,thenumberofcrossings,timeanddistanceintargetquadrantweresignificantlyincreasedintheEHOPgroups.Thebiochemicalindice
3、有良好的体内抗氧化活性。关键词:酶解物;Morris水迷宫;学习记忆;抗氧化中图分类号:TS201.4文献标志码:A文章编号:1005—9989(2013)04—0037—05ImprovingeffectofenzymatichydrolysatesfrOmoysterproteinontheabilityoflearningmemoryinmiceandtheirantioxidantactivitiesinvivoLINHai—sheng,CAOWen—hong',LUHong-yu,ZHANGChao—hua,,,QINXiao—ming,,XIEMin(1.FoodS
4、cienceandTechnologyInstitute,GuangdongOceanUniversity,Zhanjiang524088;2.KeyLaboratoryofAquaticProductAdvancedProcessingofGuangdongHigherEducationInstitutes,Zhanjiang524088;3.NationalR&DBranchCenterforShellfishProcessing,Zhanjiang524088)Abstract:Toinvestigatetheeffectsofenzymatichydrolysatesf
5、romoysterprotein(EHOPforshort)ontheabilityoflearningmemoryinmiceandtheirantioxidantactivities.EHOP1(usingneutralprotease)andEHOP2(usingalkalineprotease)wereseparatelyappliedto4week—oldmiceatadoesof5.0g/kg‘dfor4weeksandthelearningandmemoryabilitywasdeterminedusingtheMorriswatermazetests(MWM)w
6、ithTaurine(Tau)ascontrolgroupandnormalsaline(NS)group.Inordertoinvestigatetheantioxidant收稿日期:2012—11-09通讯作者基金项目:国家现代农业产业技术体系项目(CARS一48;贝类)。作者简介:林海生(1985-),男,硕士研究生。。i食品科技FOODSCIENCEANDTECHNOLOGY2013~第38卷第O4期activitieslnvivo.theSODactivityandMDAcontentinbrainandIiverwereassayed.TheMWMshowedtha
7、tEHOP1andEHOP2groupsledtoareductioninescapeIatencyf18.97+3.84Sand22.61±4.04S1comparedwiththeNSgroup.Andtheproportionofspatialstrategy,thenumberofcrossings,timeanddistanceintargetquadrantweresignificantlyincreasedintheEHOPgroups.Thebiochemicalindice
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