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时间:2020-05-18
《甜菜细胞质雄性不育相关基因的克隆表达及CMS的相关性研究.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、中国雇孝c亘搪2015,31(11):137—142ChineseAgriculturalScienceBulletin甜菜细胞质雄性不育相关基因的克隆表达及CMS的相关性研究乌日汉,斯钦巴特尔,伊六喜,田自华(内蒙古农业大学农学院,呼和浩特010019;2内蒙古农牧业科学院,呼和浩特010031)摘要:已有的研究表明,线粒体atpA基因和。87片段与作物雄性不育关系密切。为了研究atpa基因和D87片段与甜菜细胞质雄性不育之间的关系,采用同源序列法分离克隆了甜菜体内atpa和。87序列;序列分析揭示这2个片段序列在
2、不育系和相应保持系间存在差异,进一步利用半定量RT-PCR和Real—timePCR技术分析了两系的atpa基因和087片段的表达情况。结果表明,与保持系相比,atpa在不育系中发生了碱基的置换及插入;o87在不育系中缺失6个碱基,而在保持系中多了6bp的重复序列;基因表达分析也显示,不育系atpa在花蕾发育的前2个时期表达量明显低于相应的保持系,而在大花蕾期其表达量又高于相应的保持系;o87在不育系小花蕾时期表达量明显高于相应的保持系,随着花蕾的发育,该序列在不育系中的表达逐渐减弱并明显低于对应保持系中的表达量。推
3、测atpa和D87的结构变异及异常表达与甜菜细胞质雄性不育有着密切关系。关键词:甜菜;细胞质雄性不育;基因克隆;atpA;o~Cl87;半定量RT-PCR;实时荧光定量PCR中图分类号:$566.3文献标志码:A论文编号:casb14120099CloningandExpressionofCytoplasmicMaleSterilityAssociatedGenesinSugarBeetandItsRelationshiptoCMSWuRihan。,SiqinBate’er2,YiLiuxi,TianZihua(/n
4、nerMongoliaAgricultureUniversity,InstituteofAgronomy,Huhhot010019;2[nr/ErMongoliaAcademyofAgricultureandHusbandrySciences,Huhhot010031)Abstract:PreviousstudiesindicatedthatMitochondrialatpageneand087fragmentwerecloselyrelatedtomalesterilityinCrops.Inordertorese
5、archtherelationshipofatpAgenewitho87fragmentandcytoplasmicmalesterilityinsugarbeet,atpAgeneandoor/87fragmentinsugarbeetwereisolatedandclonedusinghomology—basedtechnique.Thesequencedifferencesinsugarbeetsterilelineanditscorrespondingmaintainerlineweredefinedacco
6、rdingtosequenceanalysis,expressionanalysisofatpAgeneandosC/87fragmentwasstudiedbyfurtheruseofthesemi—quantitativeRT—PCRandReal—timePCR.Theresultsindicatedthatcomparedwithmaintainerline,sterilelineatpAhadthereplacementofbasesandinsertion;6basepaweremissinginster
7、ileline087.TheexpressionanalysisdemonstratedthatexpressionofatpAinsugarbeetsterilelinewassignificantlylowerthanthatinmaintainerlinebesidesthebigflowerbudperiod,whileexpressionofosC/87insugarbeetsterilelinewasapparentlyhigherthanthatinmaintainerlinebesidesthebig
8、flowerbudperiod.Theseresultsindicatedthatstructuralvariationandabnormalexpressionpatternof口andD87hasacloserelationshipwithsugarbeetcytoplasmicmalesterility.Keywords:sugarbee
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