欢迎来到天天文库
浏览记录
ID:55573061
大小:171.33 KB
页数:2页
时间:2020-05-18
《慢性淋巴细胞白血病免疫表型及凋亡相关蛋白分析.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、里堕坠匡堂窒查!主旦簋墼鲞第期IntJLabMed,January2013,Vo1.34,No.1·25··临床检验研究论著·慢性淋巴细胞白血病免疫表型及凋亡相关蛋白分析刘越坚,郭莉,邱阳,单路娟,郭慧淑,张婵。△(1.大连医科大学附属第一医院中心实验室,辽宁大连116011;2.大连市妇产医院检验科,辽宁大连116033)。摘要:目的探讨慢性淋巴细胞白血病(CLL)免疫表型和凋亡相关蛋白表达特征及其临床意义。方法应用CD45/SSC设门方案,通过三色流式细胞术对15O例CIL患者、105例急性B淋巴细胞白血病(B-ALL)患者骨髓或外周血
2、进行免疫分型。结果150例CLI惠者均高表达CD19;HLA-DR、CD20、CD5、CD22、CD38、CD23阳性率依次为96.67、95.33、84.67、86.67、4O.67、6O.66;CD10、cCD79a、CD3、CD25、CD34、ZAP70、FMC7阳性率较低,依次为3.33%、15.33、4.67、1.33、3.33、u.43、8.67。其他抗体均无表达;与B-ALL患者相比,CLL患者CD5、CD20阳性率升高(P3、R表达率无统计学差异(P>0.05)。CLL患者凋亡相关蛋白Bcl一2、Bax阳性率分别为74.67和7.33。结论CD5、CD20、CD19是CLL特异性免疫表型,zAP70、CD38可作为预后判断指标,CII细胞Bcl一2高表达、Bax低表达,在疾病发生、发展中意义重大。关键词:白血病,淋巴细胞,慢性;免疫表型;流式细胞术DOI:10.3969/i.issn.1673—413O.2013.01.010文献标识码:A文章编号:1673—413O(2013)01-0025—02Analysisonimmunophenotypesandexp4、ressionofapoptosisproteinofchroniclymphocyticleukemiaLiuYuejian,GuoLi,QiuYang,ShanLujuan,GuoHuishu,ZhangChan(1.CentralLaboratory,FirstAffiliatedHospitalofDalianMedicalUniversity,Dalian,Liaoning116011,China;2.ClinicalLaboratory,DalianObstetricsandGynecologyHospital,Dalian,L5、iaoning116033,China)Abstract:ObjectiveToinvestigatethecharacteristicsofimmunophenotypingandexpressionofapoptosisproteininchroniclymphocyticleukemia(CII)andrelatedclinica1significance.MethodsCD45/SSCgatingandthreecolorsflowcytometrywereusedtoanalyzeimmunophenotypingandexpre6、ssionofapoptosisproteinin150patientswithCLLand105patientswithacuteB-1ymphoblasticleukemia(B—ALL).ResultsCD19washighlyexpressedinCLLpatients.ThepositiveratesofHLA—DR,CD20,CD5,CD22,CD38,CD23were96.67,95.33,84.67,86.67,40.67and6O.66,thoseofCD10,cCD79a,CD3,CD25,CD34,zAP70andFM7、C7werelow,whichwere3.33,15.33,4.67%,1.33,3.33,11.43and8.67.Theotherantigenswerenegative.ComparedwithB-AILpatients,thepositiveratesofCD5andCD20weresignificantlyhigherinCLLpatientsandthoseofCD10。CD34,CD22andcCD79aweresignificantlylower(P%0.05),buttherewerenosignificantdiffer8、encesofCD19andHLA—DRbetweenthetwogroups(P>0.05).ThepositiveratesofBcl一2andBaxwere74.67and
3、R表达率无统计学差异(P>0.05)。CLL患者凋亡相关蛋白Bcl一2、Bax阳性率分别为74.67和7.33。结论CD5、CD20、CD19是CLL特异性免疫表型,zAP70、CD38可作为预后判断指标,CII细胞Bcl一2高表达、Bax低表达,在疾病发生、发展中意义重大。关键词:白血病,淋巴细胞,慢性;免疫表型;流式细胞术DOI:10.3969/i.issn.1673—413O.2013.01.010文献标识码:A文章编号:1673—413O(2013)01-0025—02Analysisonimmunophenotypesandexp
4、ressionofapoptosisproteinofchroniclymphocyticleukemiaLiuYuejian,GuoLi,QiuYang,ShanLujuan,GuoHuishu,ZhangChan(1.CentralLaboratory,FirstAffiliatedHospitalofDalianMedicalUniversity,Dalian,Liaoning116011,China;2.ClinicalLaboratory,DalianObstetricsandGynecologyHospital,Dalian,L
5、iaoning116033,China)Abstract:ObjectiveToinvestigatethecharacteristicsofimmunophenotypingandexpressionofapoptosisproteininchroniclymphocyticleukemia(CII)andrelatedclinica1significance.MethodsCD45/SSCgatingandthreecolorsflowcytometrywereusedtoanalyzeimmunophenotypingandexpre
6、ssionofapoptosisproteinin150patientswithCLLand105patientswithacuteB-1ymphoblasticleukemia(B—ALL).ResultsCD19washighlyexpressedinCLLpatients.ThepositiveratesofHLA—DR,CD20,CD5,CD22,CD38,CD23were96.67,95.33,84.67,86.67,40.67and6O.66,thoseofCD10,cCD79a,CD3,CD25,CD34,zAP70andFM
7、C7werelow,whichwere3.33,15.33,4.67%,1.33,3.33,11.43and8.67.Theotherantigenswerenegative.ComparedwithB-AILpatients,thepositiveratesofCD5andCD20weresignificantlyhigherinCLLpatientsandthoseofCD10。CD34,CD22andcCD79aweresignificantlylower(P%0.05),buttherewerenosignificantdiffer
8、encesofCD19andHLA—DRbetweenthetwogroups(P>0.05).ThepositiveratesofBcl一2andBaxwere74.67and
此文档下载收益归作者所有