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1、_ArchivesArchVirol(1993)131:455-465Virology©Springer-Vertag1993PrintedinAustriaCharacterizationofmonoclonalantibodiestobovineentericcoronavirusandantigenicvariabilityamongQuebecisolatesBriefReportL.MichaudandS.DeaCentredeRechercheenVirologic,InstitutArmand-Frappier,Universit~duQu6bec,Laval-des-Ra
2、pides,Qu6bec,CanadaAcceptedFebruary6,1993Summary.Twentymonoclonalantibodies(MAbs)werepreparedagainsttheMebusstrainofbovineentericcoronavirus,14ofthemreactingwiththepe-plomericS(gp100)glycoprotein.Competitionbindingassaysallowedthedef-initionofatleast4distinctantigenicdomainsfortheSglycoprotein,de
3、signatedasA,B,C,andD;epitopesassociatedtoneutralizingactivitybeinglocatedinsitesA,B,andC.OneMAbdirectedtothehemagglutininHE(gp140/gp65)glycoproteininhibitedthehemagglutinatingactivityofthevirus,buthadnoneutralizingactivity.ComparisonofQuebecenteropathogenicBCVisolatesusingpolyclonalantiserumandMA
4、bsdirectedtotheSglycoproteinconfirmedtheircloseantigenicrelationship,butalsorevealedtheoccurrenceofatleastthreedistinctantigenicsubgroups.AntigenicdomainDwashighlyconservedamongBCVisolates,aswellasnon-neutralizingepitopesassignedtoantigenicdomainsAandC.TheMinnesotastrainofturkeyentericcoronavirus
5、couldbedistinguishedfromBCVisolatesbyMAbsdirectedtoepitopesofantigenicdomainC,whereashumancoronavirusHCV-OC43couldbedistinguishedbyMAbsdirectedtoepitopesofantigenicdomainA.Theporcinehemagglutinatingencephalomyelitisviruscouldbedistinguishedfromtheotherhemagglutinatingcoronavirusesbyneutralizingep
6、itopeslocatedonantigenicdomainsA,B,andC.Bovinecoronavirus(BCV)isamemberofthefamilyCoronaviridae,whichreplicatesinthedifferentiatedenterocytesofthesmallandlargeintestines,causingseverediarrheainnewborncalves[18],winterdysentery(hemorrhagicenteritis)orchronicsheddinginadultcattle[4,21].Thevirusisal
7、soresponsible456L.MichaudandS.Deaforupperrespiratorytractillnessingrowingcalves[20].Thevirioncontainsfourmajorstructuralproteins:the52knucleocapsid(N)protein,the24-26ktransmembraneormatrix(M)glycoprotein,the180-200kspi
