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1、JournalofGeneralVirology(1992),73,1725-1737.PrintedinGreatBritain1725Bovinecoronaviruspeplomerglycoproteins:detailedantigenicanalysesofSI,$2andHEJean-FrancoisVautherot,*Marie-Fran~oiseMadelaine,PascalBoireauandJacquesLaporteLN.R.A.Unit~deVirologicetImmunologieM
2、olkculaires,C.R.J.J.,DomainedeVilvert,F78352Jouy-en-JosasCedex,FranceForty-fourmonoclonalantibodies(MAbs)totheG11oindependentantigenicdomainsonS1,twoon$2,oneisolateofbovineentericcoronaviruswereusedfortheongp200andtwoonHE.Alltheneutralizinganti-S1characterizati
3、onofthepeplomerproteinsSandHE.MAbsmappedinantigenicsitesAandBandalltheFourteenoftheseMAbsreactedwithHEandtheneutralizinganti-HEMAbsinHE-B.Antigenicsiteremaining30withtheproductsoftheSgene,S1(19S1-Bwasfurthersubdividedintofoursubsites.Func-MAbs),$2(sixMAbs)andgp
4、200(fiveMAbs).S1andtionalmappingwasperformedbytestingalibraryofHEwerefoundtocarrymajorneutralizationdetermi-neutralization-resistantmutantsagainsttheneutraliz-nants,andS1appearedtoelicittheproductionoftheingMAbs.Analysisoftheirreactivityinaneutral-MAbsdisplayin
5、gthehighestneutralizingactivity.TheizationtestconfirmedtheoveralldistributionoftopographyoftheepitopeswasassessedbymeansofaepitopesinS1-BandHE-B.competitivebindingassay;the44MAbsdefinedfourIntroductional.,1990).Thesecondspike-formingprotein,HE,isadimerof125Kto1
6、40K,formedbytheassociationoftwoBovinecoronavirus(BCV)isanentericviruswhich65Kunitsbydisulphidebridges(Laporte&Bobulesco,multipliesinthedifferentiatedenterocytesofthesmall1980;Kingetal.,1985;Kienzleetal.,1990).Theroleofintestineandcolon,causingacuteenteritisinne
7、wbornHEintheagglutinationofmurineredbloodcellswascalves(StairetaL,1972;Babiuketal.,1985)andchronicdemonstratedbybiochemicalinvestigations(Kingetal.,shedding(Crouchetal.,1985)ordiarrhoea(Takahashiet1985)andbytheinhibitingactivityofmonoclonalaL,1980)inadultcows.B
8、CVparticlesconsistoflargeantibodies(MAbs)toHE(Parkeretal.,1989).HEalsosingle-strandedpositiveRNA(30kb)associatedwithahasareceptor-bindingandareceptor-destroyingactivitybasic
