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1、VimResearch,18(1990)99-1089901991ElsevierSciencePublishersB.V.0168-1702/91/%03.50ADONIS0168170291000512VIRUS00622LocalizationofmajorneutralizingepitopesontheSlpolypeptideofthemurinecoronaviruspeplomerglycoproteinSayakaTakase-Yodenr,TatekiKikuchi,StuartG.Siddell2andFumihiroTaguchirNationalInstitut
2、eofNeuroscience,NCNP,4-I-IOgawahigoshi,Koabira,Tokyo,JapanandInstituteofVirology,UniversivofWurzburgWurzburg.F.RG.(Accepted11September1990)Arecombinantbaculovirussystemhasbeenusedtoexpresstheaminoterminalhalfofthemurinecoronavirus(JHMV)peplomerglycoproteinininsectcells.Theexpressedpolypeptideisgl
3、ycosylatedandisrecognizedbyasetofmonoclonalantibodies(mAbs)specificforJHMVSprotein.ThreeofthesemAbshaveaveryhighneutralizingactivityforJHMVbutnotforotherMHVstrains.TheseresultsindicatethatJHMV-specific,majorneutralizingepitopesresideintheammotermi-nalSlsubunitofthepeplomerglycoprotein.Murinecoron
4、avirus;Peplomer;MonoclonalantibodyThepeplomerorSproteinofmurinecoronavirusesformstheprojectingspikesonthesurfaceofthevirusparticle.Thisproteinisassociatedwithattachmentofthevirustothecellsurface,thefusionofviralandcellmembranes,andtheelicitationofneutralizingantibodies(Collinsetal.,1982;Wegeetal.
5、,1984;Vennemaetal.,1990;Siddelletal.,1990).TheSproteinisalsothoughttobeimportantfordeterminingthepathogenicpotentialofthevirus(Dalzieletal.,1986;Flemingetal.,1986;Wegeetal.,1988).Correspondenceto:F.Taguchi,NationalInstituteofNeuroscience,NCNP,4-l-lOgawahigashi,Kodaira,Tokyo187,Japan.looThemurineh
6、epatitisvirus(MHV)Sproteinisaheterodimerconsistingoftwonon-covalentlyboundsubunits,theaminoterminalSlandthecarboxyterminalS2,whicharederivedbyproteolyticprocessingofanapproximately180,000kDaprecursor(Sturmanetal.,1985).Cleavageisahostcelldependenteventandisbelievedtobeoneoftheprocessingstepsneces
7、sarytoactivatethefusogeniccapacityoftheSprotein(Franaetal.,1985).InourattemptstounderstandthepathogenesisofMHV-JHMV(JHMV)infec-tions,weconsideritimportanttolocatedomainsontheSproteinwhichareimmunodominantandresponsible
