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1、JournalofGeneralVirology(1997),78,2483±2487.PrintedinGreatBritain...............................................................................................................................................................................................................SHORTCO
2、MMUNICATIONInvivoinductionofinterferon-ainpigbynon-infectiouscoronavirus:tissuelocalizationandinsituphenotypiccharacterizationofinterferon-a-producingcellsSabineRiffault,CharlesCarrat,LydiaBesnardeau,ClaudeLaBonnardie'reandBernardCharleyUnite!deVirologieetImmunologieMole!culaire
3、s,INRA,78352Jouy-en-Josascedex,FrancehumanandpigbloodleukocyteshaveshownthatNIPcellsareAlowfrequencyperipheralbloodmononuclearcellnon-phagocytic,non-adherentcellsthatlackT,Bandnatural(PBMC)subpopulation,referredtoasnaturalinter-killercelllineagespeci®cmarkers,butexpressCD4,CD36a
4、ndferon-producing(NIP)cells,isdescribedaspro-MHCclassIImolecules(Sandbergetal.,1991;Nowacki&ducinginterferon-a(IFN-a)followingcontactwithCharley,1993).Arecentstudyonpuri®ed,invitroherpesnon-infectiousviralstructures,namelyviralglyco-simplexvirus(HSV)-inducedhumanNIPcellsindicate
5、sthatproteins.Thesecellsarecharacterizedinvitroasthesecellsshareseveralphenotypicandfunctionalpropertiesnon-T,non-B,MHCclassIIMandCD4Mcells.Inthiswithdendriticcells,includingtheirabilitytopresentviralstudy,NIPcellswereanalysedinvivoafteranantigenstoTcells(Svenssonetal.,1996).int
6、ravenousinjectionofUV-inactivatedtransmiss-AfewstudieshavebeencarriedoutthatdescribeIFN-aSCiblegastroenteritisvirusinnewbornpiglets,whichinvivo.Thus,whenIFN-a}bisdetectedinthebloodduringresultedinstrongserumIFN-aproduction.Spleno-lymphocyticchoriomeningitisvirusinfectioninmice,I
7、FN-acytes,butnotPBMC,weretheIFN-aproducersinmRNA-expressingcellsoccurmainlyatthelymphoidorganvivo.Usingdoubleimmunohistochemicallabellinglevel(Sandbergetal.,1994).IFN-aSCwereaccordinglyforbothIFN-aandleukocytemarkers,weestab-detectedinthespleenorthedraininglymphnodefollowinglish
8、edthatsplenicNIPcellswerenotTorBcells.intraveno
