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1、Protein&PeptideLetters,2008,15,000-0001TheInteractionbetweenCholesterolandHumanSerumAlbumina,abaaLiuPeng*,HeMinbo,ChenFang,LiXiandZhangChaocanabSchoolofSciences,WuhanUniversityofTechnology,Wuhan430070,China;DepartmentofCardiovascularDiseases,WuhanGeneralHospital,Wuhan4
2、30070,ChinaAbstract:TheinteractionbetweencholesterolandHumanSerumAlbumin(HSA)wasstudiedbyfluorescencetechnique.AdditionofcholesterolcausesdecreasingofthefluorescenceintensityofHSAandthemechanismcanbeattributedtostaticquenching.Bothnegativeenthalpyandentropychangeindica
3、tethisbindingwasan“enthalpy-driven”reaction.Thenumberofbindingsiteanddistancebetweenresiduesandligandswerealsocalculated:n=0.98,r=3.84nm.UV–visspectrashowedHSAmoleculesunfoldedtosomeextentandthehydrophobicitywasdecreasedinthepresenceofcholesterol.Keywords:Cholesterol,q
4、uenching,thermodynamicparameter,hydrophobicity.1.INTRODUCTION2.MATERIALSANDMETHODSHumanserumalbumin(HSA)isthemostabundantpro-2.1.Materialsteininthesystemiccirculationcomprising60%inplasmaHSAandcholesterol(Fig.1)wereboughtfromSigma[1,2].Manyreportshaveshownthattherearet
5、womajorandBodiofTianjing,respectively.Trisbufferwithapurityligandbindingsiteslocatedinthesubdomainsof2Aand3Aofnolessthan99.5%andNaCl,HClwereallofanalyticalonHSA[3].Whileitsprincipalfunctionistotransportfattypurity.TheHSAwasdissolvedinTris–HClbuffersolutionacids,itisals
6、ocapableofbindinganextraordinarilybroad-1-1(0.05molLTris,0.15molLNaCl,pH7.4±0.1).Allso-rangeofdrugs[4].Muchoftheclinicalandpharmaceuticallutionswereusedwithdoublydistilledwater.interestintheproteinderivesfromitseffectsondrugphar-macokinetics.HSAhasbeenimplicatedintrans
7、port,storage,CH3andmetabolismofmanymetalions,andhasmanyphysio-logicalfunctionsasthemajorconstituentofthecirculatoryCHCH33system[5,6].HSAoftenincreasestheapparentsolubilityofCH3hydrophobicdrugsinplasmaandmodulatestheirdeliverytocellsinvivoandinvitro.CH3Cholesterolisapot
8、entmodulatorofthebulkphysicalpropertiesofbiologicalmembranes[7,8]andthepresenceofcholesterolorcholesterol-richphospho
