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ID:36642748
大小:1.08 MB
页数:41页
时间:2019-05-13
《苦参碱对脂多糖诱导的大鼠系膜细胞增殖和分泌细胞外基质的影响》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、兰州医学院硕士学位论文苦参碱对脂多糖诱导的大鼠系膜细胞增殖和分泌细胞外基质的影响姓名:张莉申请学位级别:硕士专业:儿科学指导教师:金玉2003.5.1兰州医学院硕士学位论文P514822苦参碱对脂多糖诱导的大鼠系膜细胞增殖和分泌细胞外基质的影响摘要目的了解不同浓度苦参碱(Mat6ne,M鸪对脂多糖(LPS)诱导的大鼠系膜细胞(MC)增殖及分泌细胞外基质(ECM)f拘影响,并与洛伐他汀做比较,以探讨Ma有无抗肾小球硬化的药理作用。方法采用体外培养大鼠肾小球系膜细胞(MC),随机铺于96孔板,分为空白组,LPS组(LPS10“g/m1),Ma处理组(LPSIO1.tg/rot+
2、Ma20,40,80,160,320,640prdmD,洛伐他汀处理组(LPS10ug/ml+洛伐他汀10umoVL),继续培养24h,48h,72h,以四甲基偶氮唑蓝法(MTT)检测MC的增殖情况,以台盘蓝法检测Ma对MC的细胞毒性作用,48h收集上清,以放射免疫分析法(RJA)检测MC分泌四型胶原(Col-iv)与层粘连蛋白(LN)的情况。结果经Ma处理24,48,72h后,LPS组较空白组MC增殖呈显著增加(P<0.01),Ma处理组、洛伐他汀处理组与LPS组比较、MC增殖呈显著抑STI(P48h>24h
3、,并呈剂量依赖效应(P4、5)。结论Ma可明显抑制LPS诱导的MC增殖和分泌ECM,在作用的同时无明显的细胞毒作用,并呈剂量依赖效应,有效剂量条件下优于洛伐他汀(10“mol/L)l约作用,在细胞水平上有抗肾小球硬化的药理作用。关键词苦参碱,系膜细胞,脂多糖,层粘连蛋白,IV型胶原校误英文摘要第二页末四行应为Atthe48”hours,thesecretionsofCol—IVproteinandLNofLPSgroupwerehigherthanthoseincontrolgroup◇5、错误给您带来的种种不便,敬请原谅。兰州医学院硕士学盥论文EFFECTSOFMATRINEONPROLIFERATIONANDMESANGlALMATRIXPRODUCTIoNINRATGLoMERULARMESANGIALCELLSCAUSEDBYLPSABSTRACTObjectiveThestudyaimedtoobservetheeffectsofMamne(Ma)atdifferentconcentrationsonproliferationandsecretionofextracellularmatrix(ECM)inculturedratrenalmesangi6、alcells(MC)inducedbyLipopolysaccharide(LPS),atthesametime,comparedwithLovastatin,toexploreifMatrineplaytheroleindelayofglomemlosclemsisMethodsDifferentdosagesof(20,40,80,160,320,640pg/m1)wereappliedinMCcausedbyLPSculturedfor24hs,48hsand72hs,theninhibitoryeffectsandcelltoxicreactionsofMaonM7、Cwereanalyzedwithmethylthiazolyitelrazolium(MTT)methodandcellcountingrespectively,inordertoobservetheeffectivedosage兰州医学院硕士学位论文andtime.Theexperimentsweredesignedtobethefollowingfivegroups,controlgroup,LPSgroup(LPS1012,g/m1),Magroup(LPSIOug/ml+20,40,80,160,320,
4、5)。结论Ma可明显抑制LPS诱导的MC增殖和分泌ECM,在作用的同时无明显的细胞毒作用,并呈剂量依赖效应,有效剂量条件下优于洛伐他汀(10“mol/L)l约作用,在细胞水平上有抗肾小球硬化的药理作用。关键词苦参碱,系膜细胞,脂多糖,层粘连蛋白,IV型胶原校误英文摘要第二页末四行应为Atthe48”hours,thesecretionsofCol—IVproteinandLNofLPSgroupwerehigherthanthoseincontrolgroup◇5、错误给您带来的种种不便,敬请原谅。兰州医学院硕士学盥论文EFFECTSOFMATRINEONPROLIFERATIONANDMESANGlALMATRIXPRODUCTIoNINRATGLoMERULARMESANGIALCELLSCAUSEDBYLPSABSTRACTObjectiveThestudyaimedtoobservetheeffectsofMamne(Ma)atdifferentconcentrationsonproliferationandsecretionofextracellularmatrix(ECM)inculturedratrenalmesangi6、alcells(MC)inducedbyLipopolysaccharide(LPS),atthesametime,comparedwithLovastatin,toexploreifMatrineplaytheroleindelayofglomemlosclemsisMethodsDifferentdosagesof(20,40,80,160,320,640pg/m1)wereappliedinMCcausedbyLPSculturedfor24hs,48hsand72hs,theninhibitoryeffectsandcelltoxicreactionsofMaonM7、Cwereanalyzedwithmethylthiazolyitelrazolium(MTT)methodandcellcountingrespectively,inordertoobservetheeffectivedosage兰州医学院硕士学位论文andtime.Theexperimentsweredesignedtobethefollowingfivegroups,controlgroup,LPSgroup(LPS1012,g/m1),Magroup(LPSIOug/ml+20,40,80,160,320,
5、错误给您带来的种种不便,敬请原谅。兰州医学院硕士学盥论文EFFECTSOFMATRINEONPROLIFERATIONANDMESANGlALMATRIXPRODUCTIoNINRATGLoMERULARMESANGIALCELLSCAUSEDBYLPSABSTRACTObjectiveThestudyaimedtoobservetheeffectsofMamne(Ma)atdifferentconcentrationsonproliferationandsecretionofextracellularmatrix(ECM)inculturedratrenalmesangi
6、alcells(MC)inducedbyLipopolysaccharide(LPS),atthesametime,comparedwithLovastatin,toexploreifMatrineplaytheroleindelayofglomemlosclemsisMethodsDifferentdosagesof(20,40,80,160,320,640pg/m1)wereappliedinMCcausedbyLPSculturedfor24hs,48hsand72hs,theninhibitoryeffectsandcelltoxicreactionsofMaonM
7、Cwereanalyzedwithmethylthiazolyitelrazolium(MTT)methodandcellcountingrespectively,inordertoobservetheeffectivedosage兰州医学院硕士学位论文andtime.Theexperimentsweredesignedtobethefollowingfivegroups,controlgroup,LPSgroup(LPS1012,g/m1),Magroup(LPSIOug/ml+20,40,80,160,320,
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