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1、414ISSN100723949ChinJArterioscler,Vol13,No4·实验研究·[文章编号]100723949(2005)1320420414203雷帕霉素对球囊损伤后血管平滑肌细胞表型及内膜增生的影响李玉光,许端敏,闫纯英,石永英,陈 畅,卢成志(汕头大学医学院附属第一医院心内科,广东省汕头市515041)[关键词]病理学与病理生理学; 雷帕霉素; 电镜; 血管平滑肌细胞; 内膜增生; 球囊损伤; 大鼠[摘 要] 目的 建立大鼠腹主动脉球囊损伤模型,研究雷帕霉素对血管平滑肌细胞表型和内膜增生的影响。方法 26只大鼠随机分为假手术组、球囊损伤组、羧甲基纤维素钠组和雷帕霉素
2、组。雷帕霉素组行腹主动脉球囊损伤术前3天开始每天肌肉注射雷帕霉素0.5mgPkg,术后连续14天每天肌肉注射雷帕霉素0.25mgPkg。球囊损伤14天后进行电镜观察、组织学观察和形态学分析。结果 假手术组电镜下血管平滑肌细胞呈收缩表型,球囊损伤后血管平滑肌细胞呈合成表型,雷帕霉素干预后血管平滑肌细胞电镜下表现介于两者之间。术后14天雷帕霉素组22新生内膜面积比羧甲基纤维素钠组减少36.4%(0.14±0.03mm比0.23±0.06mm,P<0.01),内膜P中膜比减少28.9%(17.7%±3.37%比28.86%±10.25%,P<0.01)。结论 以上提示雷帕霉素可以抑制血管平滑肌细
3、胞表型的改变,减轻球囊损伤后新生内膜的增生程度。[中图分类号]R363[文献标识码]AEffectofRapamycinonPhenotypeofVascularSmoothMuscleCellandArterialIntimalHyperplasiaPostBalloonInjuryinRatLIYu2Guang,XUDuan2Min,YANChun2Ying,SHIYong2Ying,CHENChang,andLUCheng2Zhi(DepartmentofCardiology,theFirstAffiliatedHospitalofShantouUniversityMedicalCo
4、llege,Shantou515041,China)[KEYWORDS]Rapamycin;ElectronMicroscope;VascularSmoothMuscleCell;NeointimalProliferation;BalloonInjury;Rats[ABSTRACT]AimToinvestigatetheeffectofrapamycinonphenotypeofvascularsmoothmusclecell(VSMC)andinti2malhyperplasiainaratmodelofrestenosispostballooninjury.MethodsTwenty2
5、sixhealthyadultmaleratswererandomlydividedintofourgroups:shamgroup(n=2),balloongroup(n=8):receivedballooninjuryonly,cellulosegroup(n=8)re2ceivedthevehiclesolution,andrapamycingroup(n=8)intramusclaradministrationofrapamycin(suspendedinsolutioncontain2ing0.2%sodiumcarboxymethylcellulose)started3days
6、beforeinjuryatadoseof0.5mgPkgandcontinuedfor14daysatadoseof0.25mgPkg.PhenotypeofVSMCwasobservedwithelectronmicroscope.Abdominalaortaswereanalyzedforintimalarea,meanintimalthickness,mediaareaandintimalPmediaarearatio.ResultsElectronmicroscopeshowedthemorphilogicchar2acteristicsofVSMCchangedfromcont
7、ractiletosyntheticafterballooninjury.VSMCshowedlessorganellesandmoremyofila2mentwhenexposedtorapamycin.NeointimalareaandintimalPmediaarearatio14daysafterinjuryshoweda36.4%and28.29%reduceintheanimalsreceivingrapam