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时间:2020-06-04
《超声辐照微泡介导雷帕霉素对兔腹主动脉球囊损伤后内膜增生影响的实验研究.pdf》由会员上传分享,免费在线阅读,更多相关内容在应用文档-天天文库。
1、志2014年9月第16卷第9inMed,Seotember2014,Vo1.16。No.9·577··实验研究·超声辐照微泡介导雷帕霉素对兔腹主动脉球囊损伤后内膜增生影响的实验研究金琳王迎春陈宏李静封岚摘要目的探讨应用超声辐照微泡增强雷帕霉素(RPM)抑制兔腹主动脉球囊损伤后内膜增生的作用。方法选取40只实验兔建立腹主动脉球囊损伤模型,将其随机分为5组,每组8只,分别为对照组、超声+微泡组、单纯RPM组、超声+RPM组及超声+微泡+RPM组。于术前及术后28d分别超声检查检测腹主动脉内一中膜厚度和全自动生化仪检
2、测血清总胆固醇(TC)、甘油三酯(TG)、谷丙转氨酶(ALT)、谷草转氨酶(AST)、肌酐(CREA)及c一反应蛋白(cRP)水平。术后28d病理检测内膜与中膜厚度比值。结果术后28d,超声发现超声+微泡+RPM组内一中膜厚度较其余组减低(P3、0.01)。结论在兔腹主动脉球囊损伤术模型中,超声辐照微泡介导RPM可抑制内膜增生,降低CRP水平,有望作为临床防治内膜增生的一种新的治疗方法。关键词超声检查;微泡;内膜增生;雷帕霉素;兔[中图法分类号]R445.1[文献标识码]AEfectofrapamycinmediatedbyultrasound-targetedmicrobubbledestructiononintimalhyperplasiawithballooninjuryinrabbitsJINLin,WANGYingchun,CHENHong,4、LIJing,FENGLanDepartmentofUltrasound,ShanghaiJiadingCenterHospital,Shanghm201800,ChinaABSTRACTObjectiveToevaluatetheeffectofrapamycinmediatedbyultrasound-targetedmicrobubbledestructiononintimalhyperplasiainabdominalaortaafterballooninjuryinrabbits.MethodsFor5、tymaleNewZealandrabbitsweredividedrandomlyintofivegroupsafterthemodelsofinjuredabdominalaortawithballoonwereprepared,includingcontrolgroup,ultrasound+microbubblegroup,rapamycingroup,ultrasound+rapamycingroupandultrasound+microbubble+rapamycingroup.Beforeand26、8dayaftertheoperation。ultrasonographywasperformedtodetecttheintimal—medialthickness(MT)andbloodsampleswere0bmined.Clinicallaboratoryassessmentincludedtotalcholesterol(TC),triglyeeride(TG),alanineaminotransferase(ALT)。aspartateaminotransferase(AST),creatinine7、(CREA),C—reactiveprotein(CRP).28dayafteroperation,theintimathickness/mediathickness(I/M)ofaortasweredetectedusingpathologicalmeans.Results28dayafteroperation,theIMTwasthinnerinultrasound+microbubble+rapamycingroupthaninothergroups(P<0.01).Therewerenosignific8、antdiferencesinTC,TG,ALT.ASTandCREAbeforeandafteroperationamongallgroups.CRPwaslowerinultrasound+microbubble+rapamycingroupthaninothergroupsandtherewassignificantdiferencebeforeandafteroperation
3、0.01)。结论在兔腹主动脉球囊损伤术模型中,超声辐照微泡介导RPM可抑制内膜增生,降低CRP水平,有望作为临床防治内膜增生的一种新的治疗方法。关键词超声检查;微泡;内膜增生;雷帕霉素;兔[中图法分类号]R445.1[文献标识码]AEfectofrapamycinmediatedbyultrasound-targetedmicrobubbledestructiononintimalhyperplasiawithballooninjuryinrabbitsJINLin,WANGYingchun,CHENHong,
4、LIJing,FENGLanDepartmentofUltrasound,ShanghaiJiadingCenterHospital,Shanghm201800,ChinaABSTRACTObjectiveToevaluatetheeffectofrapamycinmediatedbyultrasound-targetedmicrobubbledestructiononintimalhyperplasiainabdominalaortaafterballooninjuryinrabbits.MethodsFor
5、tymaleNewZealandrabbitsweredividedrandomlyintofivegroupsafterthemodelsofinjuredabdominalaortawithballoonwereprepared,includingcontrolgroup,ultrasound+microbubblegroup,rapamycingroup,ultrasound+rapamycingroupandultrasound+microbubble+rapamycingroup.Beforeand2
6、8dayaftertheoperation。ultrasonographywasperformedtodetecttheintimal—medialthickness(MT)andbloodsampleswere0bmined.Clinicallaboratoryassessmentincludedtotalcholesterol(TC),triglyeeride(TG),alanineaminotransferase(ALT)。aspartateaminotransferase(AST),creatinine
7、(CREA),C—reactiveprotein(CRP).28dayafteroperation,theintimathickness/mediathickness(I/M)ofaortasweredetectedusingpathologicalmeans.Results28dayafteroperation,theIMTwasthinnerinultrasound+microbubble+rapamycingroupthaninothergroups(P<0.01).Therewerenosignific
8、antdiferencesinTC,TG,ALT.ASTandCREAbeforeandafteroperationamongallgroups.CRPwaslowerinultrasound+microbubble+rapamycingroupthaninothergroupsandtherewassignificantdiferencebeforeandafteroperation
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