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1、提要本文对桑籽降血糖有效物质的提取、纯化、药理活性的验证等环节进行了系统试验研究，确定了合适的有效物质提取纯化方法，验证了其药理活性。黄酮、生物碱和多糖是桑资源中的是比较重要的三类活性成分。本文建立了桑籽中脂肪油、总生物碱（DNJ)、总黄酮、总多糖快速、简便的含量测定方法，对不同的提取方法进行了比较，确定了合适的提取方法；在此基础上，通过大孔树脂-聚酰胺树脂联用，确定了对总黄酮具有良好吸附的树脂型号及其分离富集工艺；又对总多糖进行醇沉、除蛋白、透析，确定了总多糖的富集工艺；然后对生物碱进行除蛋白、50%醇沉除杂、正丁醇萃取，确定了生物碱的富集工艺，最后通过单个有效部位及有效部位之间的
2、配伍确定最有效的降血糖组份。实验结果表明，桑籽中的部位经已提取分离工艺提取后，其总黄酮纯度约为52.3%,总多糖纯度为64.4%，总生物碱纯度为35.6%，经药理活性验证，生物碱和多糖具有良好的降血糖活性，为桑籽胶囊的研制和桑保健食品的开发提供了试验依据。关键词桑籽；提取；纯化；药理活性；工艺研究ResearchonExtractionandPurificationandpreIiminarypharmacologicalActivityofhypoglycemicSubstancefromMulberrySeedSpeciaIity:PharmacyofTraditionalChi
3、neseMedicineAuthor:LvZhiqiangTutor:ProfessorTIANJing-zhenAbstractWehaveresearchedonextraction,PurificationandPharmacologicalactivityaboutthistechnologyforsystemtest.Finally,wedeterminedtheprocessforindustrialproductionofMulberrySeedextractionandPurificationandPharmacologicalactivity.Firstlyweha
4、veestablishedarapidandsimplemethodofthecrudeextractofFat，totalalkaloids(DNJ),totalflavone，totalpolysaccharides,compareddifferentextractionmethodsanddeterminedtheprocessforindustrialproductionofsteiosideextractionandisolation.Onthisbasis,throughmacroporousresin-polyamideresinusedincombination,we
5、havedeterminedthetotalflavonehasgoodadsorptionresinanditsseparationprocess.Furthermore,throughalcoholprecipitationandremovingprotein,dialysis,wehavedeterminedthetotalpolysaccharideenrichmenttechnology.Next,throughtheremovalofprotein,50%alcoholprecipitationimpurity,n-butanol,wedeterminedthealkal
6、oidenrichmenttechnology.Finally,throughasingleeffectivepartandeffectivepartsofcompatibilityCompatibility,Wehavedeterminedthemosteffectivehypoglycemiccomponent.TheresultsshowthatMulberryseedinthesitealreadyextractionprocessofthetotalflavonoidsextraction,purityofabout52.3%,totalpolysaccharideswas
7、64.4%,thepurityof35.6%totalalkaloids，pharmacologicalvalidation,alkaloidsandpolysaccharideshaveexcellenthypoglycemicactivity,Thistechnologyprovidessomeevidenceformulberryseedcapsuledevelopmentandhealthfooddevelopment.Keywords:Mulbe
