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1、seriouslyeroded,holesandfragmentalstructuresfurtherincreased.SDS-PAGErevealedthat:afterpretreatment,lessthan40KDaofwheatglutenandlowmolecularweightgluteninincreased,buttherelativemolecularweightchangeofgliadinwasnotsignificant.Therelativemolecularweights
2、ofthreeproteinswereallreducedbycompoundmodification.Fourier-transforminfraredspectroscopyshowedthat:wheatglutenmainlywasβ-sheetstructure.Theβ-turnsdecreased3.23%andrandomcoilincreasedabout2.33%bysodiumsulfitepretreatment,α-helixdecreasedslightlyandrandom
3、coilincreasedbycompoundmodification.Forgliadin,theβ-turnsandβ-sheetdecreasedby6.03%and9.35%respectivelyafterpretrearment,andbothwereincreasedtoacertainextentaftercompoundmodification.Therandomcoilincreasedandthendescended.Therewerehardlyanyβ-turnsinglute
4、nin.Α-helixandβ-sheetdeclinedby3.32%and11.53%,individually,afterpretreatment.Aftercompoundmodification,theα-helixfurtherdecreased,β-sheetandβ-turnsincreasedmodestly,thechangetrendofrandomcoilinbothgluteninandgliadinwasequal.(3)Theantioxidantactivitiesofp
5、roductsmodifiedbysodiumsulfite-Alcalasewereinvestigated,suchashydroxylradicalscavengingactivity,superoxideradical2+scavengingactivity,reducingpowerandFechelatingactivity.Resultsshowedthattheantioxidantactivitieswereenhancedwiththeincreaseofthesampleconce
6、ntration,andthesmallerrelativemolecularweight,thehighertheantioxidantactivity.Escherichiacoli(E.coli)andStaphylococcusaureus(S.a)wereinhibitedtosomeextentbytheproductsofcompoundmodification.Theantibacterialactivitywaspositivelyassociatedwithsampleconcent
7、ration,whereaswasnegativelycorrelatedwiththerelativemolecularweight.Inaddition,theinhibitionwasstrongerinE.colithaninS.a.Keywords:Wheatgluten;Sodiumsulfite;Compoundmodification;Mechanism;Antioxidantactivity;AntibacterialactivityIV致谢岁月如梭，转眼间，三年的研究生求学生涯即将结
8、束，回首往昔，合肥工业大学“厚德、笃学、崇实、尚新”的校训，“勤奋、严谨、求实、创新”的校风深深影响着我。值此毕业论文完成之际，谨向所有关心、帮助我的人们表示最诚挚的感谢与最美好的祝愿。本论文是在导师钟昔阳副教授的悉心指导下完成的。从论文选题、试验设计、试验实施到论文写作及修改，每一步钟老师都给予了宝贵的意见，使我的试验和论文得以顺利完成。三年来，从钟老师身上不仅看到了渊博的专业知识、严谨的治学态度、精益求精的工作作风，还看到了豁达的人生态
