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1、安徽医科大学硕士学位论文现其浓度相对于对照组有明显升高，差异具有统计学意义（P﹤0.05），提示血浆NT-proBNP浓度变化可能是KD血液中一种特异性变化的生化指标，有望成为临床上帮助KD早期诊断的指标之一。关键词川崎病动物模型N末端脑钠肽前体诊断5安徽医科大学硕士学位论文EstablishmentofanimalmodelofKawasakidiseaseandthechangeofplasmaN-terminalpro-brainnatriureticpeptidelevelsAbstractObjectiveKawasakidisease(KD)isakindofcommo
2、npediatricautoimmunedisease,anditcancausemultisystemvasculitis,whichisleadingtodamageinthecoronaryarteryandresultinginseriouscomplicationsofcoronaryarterylesions(CAL).So,KDisbecomingastheleadingcauseofacquiredheartdiseaseinchildrenoftheworld．Currently,KDismainlybasedonclinicalsymptomsandultras
3、oundheartbeatgraphtodiagnose,lackingofspecificbiochemicalmarkersforearlydiagnosis.ThisarticlesummarizessomemethodstoestablishananimalmodelofKawasakidisease,andtomeasurethebloodbiochemicalindexes,wonderingtofindaspecificindexandhelptheearlydiagnosisofthedisease.WhilethepathogenesisofKDisunknown
4、,theestablishmentoftheanimalmodelalsoprovidesthematerialbasisforthefurtherstudy.MethodsLactobacilluscaseicellwallextract(LCWE)wasusedforimmunizationofgeneticallypredisposedmice(BALB/Cmice)toestablishanimalmodelofcoronaryarteritis.Therewere40miceasexperimentalgroupand40miceascontrolgroup.Mice（4
5、-6weeksold）wereperitonealinjectedwith0.5mlofNScontaining0.5mgLCWEorNSalonefor3days,andthen8miceineachgroupweresacrificedondays1,3,5,10and28.Bloodwascollected,andcardiactissuesofmicewereremovedandpreservedinformaldehyde.ThentissuespecimenswerestainedwithHEtoobservethepathologicchanges.After6安徽医
6、科大学硕士学位论文centrifugation,weusedthemouseN-terminalpro-brainnatriureticpeptide(NT-proBNP)ELASAkittomeasuretheconcentrationofNT-proBNPintheplasma.Ttestwasperformedonthedataobtained,comparingthechangeofNT-proBNPconcentrationbetweentwogroupsofmice.Results1.AftertheinjectionwithLCWE,theratiowhichBALB
7、/Cmicedevelopedhistologicalcoronaryarteritisofexperimentalgroupwas77.5%,andthatofcontrolgroupwas0.2.Inthelightmicroscope,weobservedonlyalittleneutrophilaccumulationalongthevascularendotheliumfirstlyinexperimentalgroupmice(day1,3),andthe
