线粒体途径在阿奇霉素诱导的哮喘大鼠气道平滑肌细胞凋亡中的作用

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1、温州医学院硕士学位论文AzithromycininducedapoptosisoftheasthmaticratsthroughthemitochondriapathwayPostgraduate：YinJuanDirector：Prof．DaiYuan—tongDir．WuCheng-yun，Dir．XiaXiao-dongAbstractOBJECTIVE：1．ToobservechangesofthemorphologyandchromatinDNAofairwaysmoothmusclecellsinvitr02．Toobservechangesofmi

2、tochondrialmembranepotential，andtheexpressionofcytochromeCandcleavedcaspase-3inairwaysmoothmusclecells．3．ToinvestigatetheinfluenceofazithromycinontheopoptosisofASMCsandthemitochondriapathway，andtoexploreitspossiblemechanisms．METHODS：20maleSprague-Dawleyratswererandomlydividedintofour

3、groupsonaverage，includingcontrolgroup(groupC)andasthmagroup(groupA0)．Inthisexperimenttheratmodelofchronicasthmawasexploited，inwhichratsweresensitizedwithovalbuminandAI(OH)3，andrepeatedlyexposedtoaerosolizedovalbumin．24hoursafterthelastchallenge，theseratswereaneshetizdwithanintraperit

4、ionealinjectionof10％chloralhydrateandweresacrificed．AirwayremodelingwasobtainedbymeasurementandcalculationofWat(totalbronchialwallarea)／Pbm(basementmembraneperimeter)andWam(smoothmusclearea)／Pbmundermicroscope．Thesmoothmusclecellswereisolatedbyprimary—explantmethodfromthebronchialtre

5、einvitro，andbetweenpassages3and8wereusedforexperiments．BronchialsmoothmusclestripswereculturedinvitroinducedbyAZMat0．1or100pg／m1．Growthactivityassaywasdetectedbycck8，thedegradationofASMCschromatinDNAwereanalyzedbyagarosegelelectrophoresis，andthemorphologicalchangeswasobservedunderIas

6、erconfocalmicroscopebystainingwithAnnexinV／PI．JC一1dyewasusedtomeasurechangesinmitochondrialmembranepotential．TheexpressionofcytochromeCandcleavedcaspase一3wasexaminedbywesternblot．RESULTS：1．Theobservationresultsoflightmicroscope：Underlightmicroscope，HE‘stainedlungtissueingroupA0showed

7、inflammatorycellsinfiltratingaroundthebronchialwallandthevascularwall．Therewerealsoevidenceofairwayepitheliaedamage,smoothmusclethickening．Thepathologicalalterationshadnotbeenseenin6温州医学院硕士学位论文groupC．2．Imageanalysisforbronchialwallthicknessandthesmoothmusclethickness：WatandWamingroup

8、Cwere31．94士5