临床医学毕业论文fk506治疗大鼠早期膜性肾病的实验研究

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1、FK506治疗大鼠早期膜性肾病的实验研宄姓名：2014年6月25日FK506治疗大鼠早期膜性肾病的实验研究作者：贾冶王晶顾华吴曼郭桥艳徐锋刘庆鑫许钟镐苗里宁【摘要】目的观察FK506治疗大鼠膜性肾病的疗效。方法复制Wistar大鼠膜性肾病模型。实验分为3组：正常组（N组），模型组（M组），FK506治疗组（FK506组），每组12只。用阳离子化中血清白蛋白制作大鼠膜性肾病模型，于正式免疫开始后2、4、6、8w检测大鼠24h尿蛋白定量;最后心脏取血，测定血清肌酐、尿素氮、白蛋白水平。处死大鼠后取肾观察肾组织病理改变;应用免疫组织化学方法

2、检测贤组织中TGFpi与NFkB的表达，应用Western印迹法测定大鼠肾组织中TGF（31蛋白表达的变化。结果M组与N组相比出现大量蛋白尿，血清白蛋白水平降低。免疫组化检测结果示M组TGFpi和NFkB的表达均高于N组，而FK506组的水平低于M组。Western印迹法显示M组及FK506组TGFpi高于N组，FK506组的水平低于M组。结论FK506能降低膜性肾病大鼠尿蛋白，提高血清白蛋白，可抑制肾组织TGFpi与NFkB的表达，用其防治大鼠早期膜性肾病有较好的疗效。【关键词】FK506;TGFp1;膜性肾病【Abstract】O

3、bjectiveToinvestigatetheeffectofFK506onthemembranousnephropathyinrat.MethodsMembranousnephropathyratswereinducedbyCBSA.Wistarratsweredividedintonormalcontrol(N),membranousnephropathy(M)，andFK506treatment(FK506)groups.Urinaryproteinexcretionwasmeasuredat2,4,6,8wfromthebe

4、ginningofimmunization.Theratbloodwasobtainedviacardiacpuncture,andthekidneyswereremoved.ThebloodsamplesweresavedformeasuringthelevelsofserumBUN,ScrandALB.Kidneyspecimenswereroutinelyprocessedforlightmicroscopy.Immunohistochemistrymethodwasperformedtoobservetheexpression

5、sofTGFpiandNFkB.TGFpproteinexpressionwasmeasuredbyWesternblot.ResultsUrinaryproteinexcretioningroupMwassignificantlyhigherthanthatingroupN.ThelevelofserumALBingroupMwaslowerthanthatingroupN.UpregulationofTGFpiandNFkBexpressionwereobservedinimmunohistochemicalfindings.Co

6、nclusionswiththatinMgroup，urinaryproteinexcretionwassignificantlydecreasedandthelevelofserumALBwassignificantlyincreasedinFK506group.ImmunohistochemistryshowedthattheexpressionsofTGFpiandNFkBweresignificantlylowerinFK506groupthanthoseingroupM.TheTGFpiproteinexpressionwa

7、ssignificantlylowerinFK506groupthanthatinMgroupbyWesternblot.ConclusionsFK506canreduceurinaryproteinexcretion，increasethelevelofALB,improvekidneypathologicalchanges，inhibitTGFpioverexpressionandNFkBactivationinrenaltissueofmembranousnephropathyrats，andcanbeusedfortreatm

8、entofearlymembranousnephropathy.【Keywords】FK506;TGFpi;Membranousnephropathy膜性肾病(membranousnephropathy，MN)是中老年人