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ID:25337884
大小:1.91 MB
页数:138页
时间:2018-11-19
《卷柏中单体化合物的抗辐射活性筛选及作用机理研究》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、英文摘要StudyonradioprotectiveeffectofMaterialBasisandMechanismfromSelaginellaTamariscinaZhuYan-hui(Pharmacochemistry)DirectedbyFengWei-shengAbstractObjective:ThisstudywasdesignedtoanalyzemonomercompoundsofSelaginellatamariscinaandevaluatetheradioprotectiveeffectonV79cellsandBALB/cmiceirradiatedwith
2、60Coγ-ray,inordertoelucidatetheradioprotectionmechanismofSelaginellatamariscina.Methods:1.WeutilizetheCCK-8AssayandGimsastainingtoobservecellproliferationandcellclonogenicsurvivalofV79cellsirradiatedwith1-8Gy60COγ-ray.V79cellsarestainedwithHoechst33258andtrypanbluetoobserveapoptosisanddeathratei
3、nducedby4,8Gyγ-irradiation.V79cellsaredetectedwithReactiveOxygenSpeciesAssayKitandPIsimplestainingtoobserveROSlevelandcellcycle.V79cellmodelisbuilttoquickscreenoutanti-radiationdrugsinvitrofromcompoundsofSelaginellaTamariscinainthisexperiment.2.Carriesonthepreliminaryscreeningofanti-radiationeff
4、ectivemonosaccharidesconstituentsfromSelaginellaTamariscinathroughtheV79cellmodel,xyloseofthemshowradioprotectiveactivitiesinvivothroughthemousemodelirradiatedby6Gy.3.Screenoutofanti-radiationactivenessfromSelaginellaTamariscinadifferentextractingpartsthroughAHH-1cellmodelirradiatedby6Gy,andeffe
5、ctivepartsofthemareverificatedthroughthemousemodelirradiatedby8Gy.Thenanalyzethechemicalconstituentof80%alcoholpartbyHPLC.ToevaluatetheradioprotectiveeffectofamentoflavonethroughtheV79cellmodelandthemousemodelirradiatedby6Gy,thecellviability,cellclonogenicsurvival,cellcycleand30-daysurvivalratea
6、redetected.Results:1.WefindthatV79celldeathrateincreasewith60COγ-raydoes-dependentmanners.Alongwiththeincreaseofradiationtimeordose,thecellviabilitygraduallydecrease.Exposureto4,8GyinducecelldeathofV79cellswithcellularaswellasnuclearswellingandcytosolicvacuolation.Neithernuclear-3-卷柏中单体化合物的抗辐射活性
7、筛选及作用机理研究shrinking,apoptosisbodynorsub-G1phasearedetected.Inaddition,ROSlevelsandG2arrestsignificantlyincrease,suggestingthatV79cellsirradiatedby4,8Gydieviaoncosisratherthanapoptosis.2.Thereareglucose,xylose,fructose,arabino
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