资源描述：
《卷柏中单体化合物的抗辐射活性筛选及作用机理研究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、英文摘要StudyonradioprotectiveeffectofMaterialBasisandMechanismfromSelaginellaTamariscinaZhuYan-hui(Pharmacochemistry)DirectedbyFengWei-shengAbstractObjective:ThisstudywasdesignedtoanalyzemonomercompoundsofSelaginellatamariscinaandevaluatetheradioprotectiveeffectonV79cellsa
2、ndBALB/cmiceirradiatedwith60Coγ-ray,inordertoelucidatetheradioprotectionmechanismofSelaginellatamariscina.Methods:1.WeutilizetheCCK-8AssayandGimsastainingtoobservecellproliferationandcellclonogenicsurvivalofV79cellsirradiatedwith1-8Gy60COγ-ray.V79cellsarestainedwithHoec
3、hst33258andtrypanbluetoobserveapoptosisanddeathrateinducedby4,8Gyγ-irradiation.V79cellsaredetectedwithReactiveOxygenSpeciesAssayKitandPIsimplestainingtoobserveROSlevelandcellcycle.V79cellmodelisbuilttoquickscreenoutanti-radiationdrugsinvitrofromcompoundsofSelaginellaTam
4、ariscinainthisexperiment.2.Carriesonthepreliminaryscreeningofanti-radiationeffectivemonosaccharidesconstituentsfromSelaginellaTamariscinathroughtheV79cellmodel,xyloseofthemshowradioprotectiveactivitiesinvivothroughthemousemodelirradiatedby6Gy.3.Screenoutofanti-radiation
5、activenessfromSelaginellaTamariscinadifferentextractingpartsthroughAHH-1cellmodelirradiatedby6Gy,andeffectivepartsofthemareverificatedthroughthemousemodelirradiatedby8Gy.Thenanalyzethechemicalconstituentof80%alcoholpartbyHPLC.Toevaluatetheradioprotectiveeffectofamentofl
6、avonethroughtheV79cellmodelandthemousemodelirradiatedby6Gy,thecellviability,cellclonogenicsurvival,cellcycleand30-daysurvivalratearedetected.Results:1.WefindthatV79celldeathrateincreasewith60COγ-raydoes-dependentmanners.Alongwiththeincreaseofradiationtimeordose,thecellv
7、iabilitygraduallydecrease.Exposureto4,8GyinducecelldeathofV79cellswithcellularaswellasnuclearswellingandcytosolicvacuolation.Neithernuclear-3-卷柏中单体化合物的抗辐射活性筛选及作用机理研究shrinking,apoptosisbodynorsub-G1phasearedetected.Inaddition,ROSlevelsandG2arrestsignificantlyincrease,sug
8、gestingthatV79cellsirradiatedby4,8Gydieviaoncosisratherthanapoptosis.2.Thereareglucose,xylose,fructose,arabino
