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1、淫羊藿提取物的雌激素样作用研究第30卷第3期2009年6月西安交通大学(医学版)JournalofXi'anJiaotongUniversity(MedicalSciences)Vo1.30No,3Jun.2009淫羊藿提取物的雌激素样作用研究于燕,颜虹,胡森科,张敬华(西安交通大学医学院公共卫生系,陕西西安710061)摘要:目的评价淫羊藿提取物的雌激素样作用.方法采用小鼠子宫增重实验,雌激素受体阳性细胞系(MCF一7)细胞增殖及拮抗实验,评价淫羊藿及淫羊藿苷的雌激素样作用及其可能机制.结果与阴性对照组比较,淫羊藿及淫羊藿
2、苷能使子宫系数由0.097±0.008明显增加至0.156±0.018及0.152±0.016,且用药前,后动物体重由(18.9±1.3)g分别增至(19.8±1.3)g及(2O.03±1.3)g,增幅明显高于阴性列照组(P<0.05);淫羊藿苷可使血清雌激素E山(132.22±11.45)ng/L增高至(147.73±12.43)ng/I,FSH水平由(50.15±3.36)t~g/L降低至(44.47±2.46)g/!(P<O.05),但淫羊藿组雌激素E.水平无明显改变;在24,48,72h,淫羊藿及淫羊藿苷
3、对MCF一7细胞的增殖均打叫显的促进作用(P<0.05),分别由0.238±0.043,0.387±0.051,0.563±0.083增至0.362±0.045,0.521±O.046,0.648±0.043及0.324±0.043,0.465±0.034,0.622±0.079;72h加入ICI后,细胞增殖明显受到抑制,细胞增『『【}-半分别降至99.461,94.434.结论淫羊藿及淫羊藿营具有雌激素样作用,且其促细胞增殖作用可能是通过雌激素受休(ER)介导产生的.关键词:淫羊藿;提取物;雌激素样作用中图分类号:R
4、284;R285.5文献标志码:A文章编号:1671—8259(2009)03037304Studyontheestrogen—likeeffectsofEpimediumextractiveYANHong,HUSenke,ZHANGJing—huaMedicalSchoolofXi'anJiaotongUniversity,Xi'an710061.China)ABSTRACT:ObjectiveToinvestigatetheestrogen.1ikeeffectsofEpimediumextractive.Methods
5、ByusingtheuterusgrowthtestinmiceandtestofMCF一7proliferationandaddingestrogenreceptorantagonistIcariin,weevaluatedtheestrogeniceffectsofEpimediumandIcariinandthepossiblemechanism.ResultsEpimediumandIcariinincreasedsignificantlytheuteruscoefficientinmicefrom0.097±0.0
6、08to0.156±0.018and0.152±0.016,respectively:theweightincreasedsignificantlyfrom(18.9±1.3)gto(19.8±1.3)gand(20.03±1.3)g(P<O.05).IcariinincreasedsignificantlytheserumlevelofE2from(132.22±11.45)ng/Lto(147.73±】2.43)ng/LanddecreasedFSHlevelfrom(50.15±3.36)g/Lto(44.47±
7、2.46)g/L(P<o.05),buttherewasnosignificantdifferenceinEpimediumgroup.ThercwasobviouslyenhancedproliferationinMCF一7in24,48and72h(P<0.05),from0.238±0.043,0.387±0.051and0.563±0.083to0.362±0.045,0.521±0.046and0.6484-_0.043aswellas0.324土0.043,0.465±0.034and0.622±0.
8、079,respectively.However,thecellproliferationwasinhibitedsignificantlybyIcariinaddedin72h-theproliferationratiodecreasedto99.461%and94.434%?respe
