基因操作原理 基因操作原理-练习题

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1、基因操作原理1.Geneticengineeringhassuccessfullytransferredgenesfromeukaryoticcellsinto:A)Bacteria;B)plants;C)animals;D)allofabove2.Thegenemanipulationwasbornin:A)1970;B)1972;C)1973;D)19763.Thefirstfunctionalgeneproductfromachemicallysynthesizedgeneis:A)Growthhonmore;B)

2、somatostatin;C)insullin;D)interferon4.Physicalprotectionhasfourlevels,thehighestlevelis:A)P1;B)P2;C)P3;D)P45.Whichoneoffollowingarethebasictypeofphageλvectors?A)Insertionalvectors;B)replacementvectors;C)bothAandB;D)noneoftheabove6.Themainadvantageofcosmidoverboth

3、plasmidandphageis:A)CosmidcanbeusedforcloninglargerpiecesofforeignDNA;B)CosmidisdsDNAvector;C)Cosmidhascossite;D)Cosmidcanbepackedinvitro7.WhichoffollowingcharactersisnottheadvantageofM13seriesvector?A)Itcanbereplicatedviaadouble-strandedcircularDNA(RF)intermedia

4、teB)BothRFandsingle-strandedDNAwilltransfectcompetentE.colicellstoyieldeitherplaquesorinfectedcolonies.C)Therearenopackagingconstraints;D)Allofabove1.Thephasemidshavebeendevelopedbycontaining:A)ApartofthelacZgenewithpBR322B)ApartofthelacZgenewithM13C)ApartoftheM1

5、3genomewithpUC8D)ApartoftheλgenomewithpUC82.Whichofthefollowingisnotamethodtoobtainfull-lengthcDNAclone?A)CAPture;B)oligo-capping;C)primerextension;D)5’-RACE3.CellstransformedwithpBR322carringforeignfragment(nopromoteractivity)inthepromoterregionofTcRgene,itsgeno

6、typeis:A)ApRTcR;B)ApSTcR;C)ApRTcS;D)ApSTcS4.Western-blotscreeningforagenedependson:A)Theclonegeneexpressed;B)Thepurifiedantibodybindsonlytothetargetprotein;A)ThetargetproteinisDNAbindingprotein;B)BothAandB1.PCRscreeningbelongsto:A)Sequence-dependentscreeningB)Imm

7、unologicalscreeningC)FunctionalscreeningD)Allofabove2.Recombinationalprobemethodforscreeningrecombinantclonesis:A)Baseduponexpressingvector;B)Baseduponexpressionoftheclonedgene;C)BaseduponhomologousrecombinationinE.coliD)Noneoftheabove3.Ifweknowthesequenceofamous

8、eproteinandwanttoexpressitinE.coli,thebestchoiceforfirststepis:A)IsolatemousegenomicDNA,thenusePCRtoamplifythetargetgene;B)IsolatemousecelltotalRNA,thenuseRT-P