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1、瑞舒伐他汀对ox-LDL诱导的TF表达的影响及机制研究吕慎昌颜新峰高爱玲(山东省莱芜市妇幼保健院山东莱芜271100)【】目的:探讨瑞舒伐他汀对氧化修饰低密度蛋白(ox-LDL)诱导的人单核-巨噬细胞组织因子(TF)表达的影响机制。方法:采用体外密度离心法分离健康人单核细胞培养并培养到第4代,采用佛波脂(160nmol/L)对巨噬细胞进行诱导24h后用于实验,根据实验要求分为空白组、ox-LDL组、瑞舒伐他汀组,采用逆转录聚合酶链式反应(RT-PCR)测定各组的TFmRNA、TF蛋白、人单核-巨噬细胞(LOX-lmRNA
2、)的表达情况。结果:TF基因的RT-PCR产物溶解曲线温度峰值为82.5°C,溶解曲线未见任何切迹,曲线光滑锐利,证实TF基因扩增产物特异性强,无异常扩增现象。三组实验标木的LOX-lmRNA、TFmRNA、TF蛋白表达差异显著(P<0.05);组间两两比较,LOX-lmRNA、TFmRNA、TF蛋白在三组间的差异为ox-LDL组>瑞舒伐他汀组>空白组(P<0.05)。结论:瑞舒伐他汀可以通过下调TFmRNA、LOX-lmRNA表达水平从而达到TF蛋白的表达,可以有效降低急性冠脉事件的发牛.率。【
3、关键词】瑞舒伐他汀;氧化修饰低密度蛋白;人单核-巨噬细胞组织因子;影响机制【1R541.4【】A【11004-6194(2015)01-0001-02Studyontheeffectofrosuvastatinonexpressionofox-LDLinducedbyTFanditsmechanism【Abstract】Objective:Studyofrosuvastatinonoxidizedlowdensitylipoprotein(ox-LDL)inducedhumanmonocyte-macrophagetis
4、suefactor(TF)expressionoftheimpactmechanism.Methods:Isolationandcultureofhealthhumanmononuclearcellsandculturedtothefourthgenerationofinvitrousingdensitycentrifugation,byphorbolester(160nmol/L)onmacrophageswereusedfortheexperimentaftertheinductionof24h,accordingt
5、otheexperimentalrequirementsaredividedintoblankcontrolgroup,ox-LDLgroup,rosuvastatin,reversetranscriptionpolymerasechainreaction(RT-PCR)weremeasuredTFmRNA,TFprotein,humanmonocytemacrophages(LOX-lmRNA)expression.Results:TheproductofRT-PCRTFgeneofthedissolutioncurv
6、eofthepeaktemperatureis82.5DEGC,anynotchnodissolutioncurvesmooth,sharp,confirmedthattheTFgeneamplifiedproductspecificity,noabnormalamplificationphenomenon.ThethreegroupsoftheexperimentalspecimensofLOX-lmRNA,TFmRNA,TFproteinexpressionweresignificantlydifference(P&
7、lt;0.05);thecomparisonbetweentwotwogroups,thedifferenceofLOX-lmRNA,TFmRNA,TFproteininthreegroupsox-LDLgroup>rosuvastatingroup>blankgroup(P<0.05).Conclusion:Rosuvastatincanthroughthedown-regulationofTFmRNALOX-lmRNAexpression,soastoachievethelevelofTFprote
8、in,caneffectivelyreducetheincidenceofacutecoronaryevents.【Keywords】RuiShuVatatin;oxidizedlowdensitylipoprotein;mononuclearmacrophagetissuefactor;influencemecha